食品科学 ›› 2005, Vol. 26 ›› Issue (2): 38-42.

• 基础研究 • 上一篇    下一篇

微生物结构的动力损伤及其导致的菌体失活

 丁文波, 张绍英     

  1. 中国农业大学食品科学与营养工程学院; 中国农业大学食品科学与营养工程学院
  • 出版日期:2005-02-15 发布日期:2011-09-19

Dynamic Damage Mechanism on Microbiology’s Structure and Pasteurization

 DING  Wen-Bo, ZHANG  Shao-Ying   

  1. College of Food Science and Nutritional Engineering, China Agriculture University
  • Online:2005-02-15 Published:2011-09-19

摘要: 研究了枯草芽孢杆菌细胞的动力损伤与菌体失活的关系。结果表明:(1)动力作用导致的细菌亚结构损伤可造成细菌失活。(2)较低的动力作用即可造成枯草芽孢杆菌细胞的整体破碎和亚结构的致死性损伤。(3)微孔均质阀对细菌表层结构的破坏形式表现为细胞壁擦伤和细胞膜破裂。(4)较低强度的动力作用即可造成细菌的破碎和致死性亚结构损伤,但使细菌全部破碎则需多次处理和高强度的动力作用,故致死性亚结构损伤的作用机理有待进一步研究。(5)动力作用对细菌细胞内部结构的损伤,有赖于通过电镜观察细胞超薄切片来进一步研究。

关键词: 枯草芽孢杆菌, 细胞结构, 高压均质, 动力损伤, 活性

Abstract: The relationship between dynamic damage and life activity of Bacillus subtilis was studied. The results showed: (1) Submicroscopic structure damage caused by dynamic actions could make the bacteria lose life. (2) Lower dynamic actions could make the Bacillus subtilis completely-break and its submicroscopic structure fatally damage. (3) The effects of micro-hole valve of homogenizer on bacteria’s surface structure showed cell wall friction damage and cell membrane breakage. (4) The whole- breakage of bacteria need many times repeatedly handling with high dynamic action though the breakage and death caused by submicroscopic damage could occur in rather low dynamic action. The mechanism of submicroscopic structure to cause fatal damage should need further. (5) The effect of dynamic action on bacteria’s inner-structure should need further observing cell’s super fine slices through Transmission Electron Microscope study.

Key words: Bacillus subtilis, cell’s structure, high pressure homogenization, dynamic damage, life activity