食品科学 ›› 2005, Vol. 26 ›› Issue (2): 64-67.

• 基础研究 • 上一篇    下一篇

木瓜蛋白酶降解壳聚糖产物分子量分布模拟研究(Ⅱ)——算法验证

 黄永春, 伍时华, 李琳, 李军生, 容元平   

  1. 广西工学院生物与化学工程系; 华南理工大学食品与生物工程学院
  • 出版日期:2005-02-15 发布日期:2011-09-19

Simulation for Molecular Weight Distribution of Chitosan after Degradation by Papain (Ⅱ) ——Validatingt heA rithmeticR eliability

 HUANG  Yong-Chun, WU  Shi-Hua, LI  Lin, LI  Jun-Sheng, RONG  Yuan-Ping   

  1. 1.Department of Biological and Chemical Engineering, Guangxi University of Technology; 2.College of Food and Biological Engineering, South China University of Technology
  • Online:2005-02-15 Published:2011-09-19

摘要: 壳聚糖降解产物分子量分布的计算机模拟是其最优化控制的基础,而模拟算法的可靠性又是其得以应用与否的关键。为了验证“简化-复原”模拟算法的可靠性,首先用木瓜蛋白酶降解了四种不同规格的壳聚糖,通过GPC测定了不同降解程度下的降解产物分子量分布,然后通过计算机模拟相同降解条件下产物分子量分布。结果表明,“简化-复还”算法能很好地反映壳聚糖酶解产物分子量分布。该算法可以作为降解产物分子量分布优化控制的基础。

关键词: 壳聚糖, 降解, 分子量分布, 模拟, 验证

Abstract: In order to testify the effect and reliability of“ simplified-reverted” simulation method, the molecular weight distribution (MWD) of several chitosan samples with different degree of deacetylation (DD) after degradation was measured by gel-permeation chromatography(GPC), and the MWD of the same samples was simulated by“ simplified-reverted” method. It was shown that the method mentioned above could effectively predict the trend of MWD of the enzymatic degradation products of chitosan. The method could be employed as the predictive approach and the basis of optimal control on the MWD of chitosan after degradation by papain.

Key words: chitosan, degradation, molecular weight distribution, simulation, validation