食品科学 ›› 2005, Vol. 26 ›› Issue (2): 68-70.

• 基础研究 • 上一篇    下一篇

不同注射处理对牛肉剪切力和肌原纤维小片化指数的影响

 黄明, 周光宏, 徐幸莲, 赵莲   

  1. 南京农业大学农业部农畜产品加工与质量控制重点开放实验室
  • 出版日期:2005-02-15 发布日期:2011-09-19

Effects of Injection Treatments on Beef Warner-Bratzler Shear Force and Myofibrillar Fragmentation Index

 HUANG  Ming, ZHOU  Guang-Hong, XU  Xing-Lian, ZHAO  Lian   

  1. Key Laboratory of Agricultural and Animal Products Processing and Quality Control, Ministry of Agriculture, Nanjing Agriculture University
  • Online:2005-02-15 Published:2011-09-19

摘要: 本文研究了不同注射处理对牛肉在成熟过程中剪切力,肌原纤维小片化指数(MFI)的影响。3头改良黄牛(鲁西黄牛×犁木赞)在屠宰厂按照标准的屠宰工艺屠宰并预冷20h后取样,将样品给予7个不同的处理,每个处理分别按样品重10%注射蒸馏水(作为对照)、200mmol/L CaCl2、200mmol/L EGTA、200mmool/L ZnCl2、0.2mg/ml亮肽素、0.2mg/ml亮肽素+1%Triton X-100、1%Triton X-100,处理后将样品分别成熟3、8、16d后测一些指标变化。结果发现注射外源性钙激活酶抑制剂(亮肽素,ZnCl2)显著降低了牛肉的成熟速度,使牛肉剪切力增加,MFI降低;而注射CaCl2则相反,且注射CaCl2后牛肉成熟4d时的剪切力和对照组成熟16d时的差异不显著(p>0.05)。

关键词: 牛肉, 注射处理, 剪切力, 肌原纤维小片化指数

Abstract: This experiment was conducted to research into the influence of different treatments on beef Warner-Bratzler shear force and myofibrillar fragmentation index (MFI). Three crossbred yellow cattle (Luxin× Limusin) were slaughted according to standard slaughting practices. After being chilled for 20h, samples were made from carcasses and were injected (10% of weight) with distilled water(as control) or with 200 Calcium chloride, 200mmol/L EGTA, 200mmol/L Znic chloride, 0.2mg/ml Leupeptin, 0.2mg/ml Leupeptin plus 1% Triton X-100, and 1% Triton X-100 respectively. The result indicated that exogenous calpains inhibitors, Leuptptin and Znic chloride have slowed beef aging process significantly, and made beef with higher Warner-Bratzler shear force (WBS) and lower Myofibrillar fragmentation index. On the contrary, however, calcium chloride injection greatly shortened beef aging period, and the shear force value of beef injected with calciun chloride and aged for 4 d was not significantly different with that of control aged for 16 d.

Key words: beef, injection-treatment, Warner-Bratzler shear force, myofibrillar fragmentation index