食品科学 ›› 2005, Vol. 26 ›› Issue (2): 75-78.

• 基础研究 • 上一篇    下一篇

α-转移葡萄糖苷酶粗酶液的酶学性质

 毕金峰, 魏宝东   

  1. 中国农业科学院农产品加工研究所农业部农业核技术与农产品加工重点实验室; 沈阳农业大学食品学院
  • 出版日期:2005-02-15 发布日期:2011-09-19

The Impurified Enzyme Properties of Alpha-transglucosidase

 BI  Jin-Feng, WEI  Bao-Dong   

  1. 1.Institute of Agro-Food Science and Technology, Chinese Academy of Agricultural Sciences Key Laboratory of Agricultural Nuclear Technology and Agro-Food Processing of MOA;2.College of Food Science, Shenyang Agricultural University
  • Online:2005-02-15 Published:2011-09-19

摘要: 用实验室制备的α-转移葡萄糖苷酶粗酶液进行酶反应,反应至一定时间后,生成较高含量的低聚异麦芽糖。该粗酶液的最适pH值为5.0,最适反应温度为55℃,不同金属离子对粗酶液酶活的影响不同。正交试验确定粗酶液的最佳反应pH值为5.15,温度为55℃,反应时间为3h。

关键词: &alpha, -转移葡萄糖苷酶, 粗酶, 性质

Abstract: There was a high isomaltooligosaccharide concentration in reaction solution after a period of catalytic reaction by impurified enzyme of α-transglucosidase. The optimum pH value and temperature of the impurified enzyme were 5.0 and 55℃ respectively. The effect of different metal ions on enzyme activity was different. The results of orthogonal experiment showed that the optimum catalytic reaction conditions were pH 5.15, 55℃ and 3h(reaction time).

Key words: α-transglucosidase, coarse enzyme, property