嗜碱芽孢杆菌产环糊精葡萄糖基转移酶发酵条件的优化

食品科学 ›› 2005, Vol. 26 ›› Issue (2): 122-126.

嗜碱芽孢杆菌产环糊精葡萄糖基转移酶发酵条件的优化

曹新志, 金征宇

江南大学食品学院

出版日期:2005-02-15 发布日期:2011-09-19

Optimization Study on Cyclodextrin Glycosyltransferase Production by Bacillus alkalophilus

CAO Xin-Zhi, JIN Zheng-Yu

College of Food Science and Technology, Southern Yangtze University

Online:2005-02-15 Published:2011-09-19

摘要/Abstract

摘要： 对一株嗜碱芽孢杆菌产环糊精糖基转移酶的发酵条件进行了研究。利用单因素试验和正交试验获得该菌株产环糊精糖基转移酶的最佳条件为:接种量3%;培养温度30℃;pH10.5;发酵培养基的组成为玉米粉2%,酵母膏1.5%,玉米浆5%;250ml三角瓶装液量为30ml;270r/min振荡培养3d,其发酵液酶活可达5400U/ml左右。10L罐发酵时酶活可达5820U/ml。

关键词: 嗜碱芽孢杆菌, 环糊精糖基转移酶, 发酵条件, 优化

Abstract: Conditions of cyclodextrin glycosyltransferase (CGTase) production by Bacillus alkalophilus12-7 were studied. The optimal fermentation conditions for cyclodextrin glycosyltransferase production were determined by a single-factorial experiment and orthogonal experiment. The results showed that the optimal conditions were as follows: amount of inoculum is 3%; incubation temperature 30℃; pH 10.5. The compositions of fermentation medium were maize powder 2%, yeast extract 1. 5% and corn steep liquor 5%. The load of 250ml-flask was 30ml and the shaking incubation took 3d at 270r/min and the enzyme activity could reach to 5400 U/ml.In 10L-fermentor the enzyme activity could reach as high as 5820U/ml.

Key words: Bacillus alkalophilus, cyclodextrin glycosyltransferase, fermentation conditions optimization

曹新志, 金征宇. 嗜碱芽孢杆菌产环糊精葡萄糖基转移酶发酵条件的优化[J]. 食品科学, 2005, 26(2): 122-126.

CAO Xin-Zhi, JIN Zheng-Yu. Optimization Study on Cyclodextrin Glycosyltransferase Production by Bacillus alkalophilus[J]. FOOD SCIENCE, 2005, 26(2): 122-126.

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