食品科学 ›› 2005, Vol. 26 ›› Issue (2): 127-130.

• 工艺技术 • 上一篇    下一篇

胰蛋白酶水解富集米胚芽中γ-氨基丁酸的研究

 张晖, 姚惠源   

  1. 江南大学食品学院
  • 出版日期:2005-02-15 发布日期:2011-09-19

Study on Accumulation of γ-Aminobutyric Acid in Rice Germ by Hydrolysis of Trypsinase

 ZHANG  Hui, YAO  Hui-Yuan   

  1. College of Food Science and Technology, Southern Yangtze University
  • Online:2005-02-15 Published:2011-09-19

摘要: 研究了胰蛋白酶水解米胚蛋白富集γ-氨基丁酸(GABA)的工艺,通过单因素实验和正交实验等确定了最佳的米胚芽水解并富集GABA的工艺条件,即料水比为1/14,温度为40℃,加酶量为4080U/100g米胚芽,反应7h后,加入1.5g/100ml的米胚芽,40℃继续反应6h,GABA产量可达2.26g/100g,比未富集前的0.028g/100g提高了80倍。

关键词: 大米胚芽, 氨基丁酸, 胰蛋白酶, 富集

Abstract: The accumulation condition of γ-aminobutyric acid in rice germ by hydrolysis of tripsinase were studied. The optimum conditions were: temperature 45℃, pH8.0, and the ratio of rice germ to water 1/14. After 7h of hydrolyzing, 1.5g/ 100ml of rice germ were added to the solution and reacted continually for 6h. The content of γ-GABA in rice germ could reach as high as 2.26g/100g from the original 0.028g/100g.

Key words: γ-aminobutyric acid, rice germ, trypsinase, accumulation