关键词: 决明子, 脂类, 提取, 测定

Abstract: The lipids were extracted by using methanol and chloroform after comminuting the seeds of Cassia Tora in the environment of dry ice. Different components of lipids separated with thin layer chromatogram (TLC) were methylated and determined by GC equipped with 100m×0.25mm capillary column. The results indicated that the lipids were composed of triglyceride (TG), cholesterol ester (CE) and free fatty acids (FFA). Among them TG, a main component, was 74.689%, while FFA and CE were 17.840% and 7.470% respectively. This difference was resulted from 16:0, 18:0, 18:1C, 9c12c18:2 and 18: 3n3. Saturated fatty acids (SFA) in CE was higher than that in TG and FFA, 30.8315%,26.469% and 24.221% respectively, in which 14:0, 20:0, 21:0,22:0 and 24:0 in CE were all higher than that in TG and FFA except for 16:0 (12.148%,16.287% and 17. 613% respectively). Polyunsaturated fatty acids (PUFA) had the highest concentration in TG, CE and FFA, and were higher in FFA and TG than in CE, 46.156%, 45.107% and 41.042% respectively. This difference was mainly caused by different content of 9c12c18:2 (44.252%,43.598% and 38.833% in FFA, TG and CE respectively). In conclusion, lipids in FFA were similar to those in TG, but differed from those in CE.

Key words: Cassia Tora, lipids, extraction, determination