食品科学 ›› 2005, Vol. 26 ›› Issue (4): 73-75.

• 基础研究 • 上一篇    下一篇

双歧杆菌免疫血清的制备及性质分析

 袁耀武, 张伟, 马雯, 李英军, 林杨   

  1. 河北农业大学食品科技学院
  • 出版日期:2005-04-15 发布日期:2011-09-19

Preparation and Analysis of Bifidobacterium Immune Serum

 YUAN  Yao-Wu, ZHANG  Wei, MA  Wen, LI  Ying-Jun, LIN  Yang   

  1. College of Food Science and Technology, Agriculture University of Hebei
  • Online:2005-04-15 Published:2011-09-19

摘要: 通过动物免疫方法制备了长双歧(B.longum)和两歧双歧杆菌(B.bifidum)的免疫血清,经ELISA检测,血清效价达到1:1280。用此血清和乳制品中常见细菌反应,未见明显交叉反应,这一结果为双歧杆菌发酵乳制品中双歧杆菌的血清学检测提供了参考。

关键词: 双歧杆菌, 免疫血清, ELISA

Abstract: In this experiment,we were prepared B.Longum and B.Bifidum immune serum by inoculating animals, with the titer of the serums to reach 1:1280 by Enzyme-linked Immunosorbent Assay (ELISA) method. The serums reacted highly specifically with Bifidobacteria, and hardly reacted with other familiar bacteria in dairy products. The result provided a base for serological detecting of Bifidobacterium in dairy products.

Key words: Bifidobacterium, immune serum, ELISA