食品科学 ›› 2005, Vol. 26 ›› Issue (4): 222-225.

• 分析检测 • 上一篇    下一篇

薄层分离—分光光度法测定大叶紫薇叶中的总三萜含量

 纵伟, 夏文水, 崔宝良   

  1. 江南大学食品学院; 无锡杰西医药科技有限公司
  • 出版日期:2005-04-15 发布日期:2011-09-19

Determination of Total Triterpene in Lagerstroemia specious L.by Thin Layer Chromatography-spectrophotometry

 ZONG  Wei, XIA  Wen-Shui, CUI  Bao-Liang   

  1. 1. School of Food Science,Southern Yangtze University; 2.Jiexi Pharmaceutical Technology Co.Ltd.
  • Online:2005-04-15 Published:2011-09-19

摘要: 采用薄层色谱分离-分光光度法测定大叶紫薇叶中的总三萜含量.研究了展开剂的组成,显色反应的条件,并对该法的精密度、准确性及稳定性等进行了探讨.结果表明,适宜的展开剂组成为氯仿:丙酮=4:1;显色反应的适宜条件为:5%香草醛醋酸溶液0.3ml,高氯酸1ml,60℃反应15min,该法具有较好的精密度、准确性及稳定性,测定结果同HPLC法分析结果相比无明显差异,可用于大叶紫薇叶中的总三萜含量的测定。

关键词: 大叶紫薇, 总三萜, 薄层分离, 分光光度法

Abstract: The procedure of thin layer chromatography-spectrophotometry analysis of total triterpene in Lagerstroemia specious L. leaf was investigated in this paper. The results showed that the accuracy of determination was high, processing with the following procedure: acetone (4:1 in volume ratio) as developer,0.3ml of 5% vanillin/glacial acetic acid added to 1ml perchloric acid solution, heating 15 minutes at 60℃, and the absorbance measured at 542 nm after being added 5ml glacial acetic acid and cooling. The recovery of the procedure was 100.22% and RSD 2.05%(n=5).The result showed no sharp difference with HPLC result.

Key words: Lagerstroemia specious L, total triterpene, thin layer chromatography, spectrophotometry