食品科学 ›› 2005, Vol. 26 ›› Issue (5): 204-207.

• 分析检测 • 上一篇    下一篇

应用胶体金试纸条快速检测赭曲霉毒素A的研究

 赖卫华, 熊勇华, 陈高明, 许杨   

  1. 南昌大学食品科学教育部重点实验室,江西中德大地生物工程有限公司
  • 出版日期:2005-05-15 发布日期:2011-09-19

Preparation of Colloidal Gold Strip for Rapid Detection of Ochratoxin A

 LAI  Wei-Hua, XIONG  Yong-Hua, CHEN  Gao-Ming, XU  Yang   

  1. 1.The Key Laboratory of Food Science, Nanchang University, Ministry of Education;2.Jiangxi Zodears Biotechnology Co. Ltd.
  • Online:2005-05-15 Published:2011-09-19

摘要: 本文介绍了一种快速检测赭曲霉毒素A的胶体金试纸条的研制方法,其中包括胶体金的生产、金标抗体的制备、试纸条的组装和测试等步骤。测试结果表明赭曲霉毒素A快速检测试纸条的检测限为10ng/ml,检测时间为10min,加上使用方便,经济适用,使之适合于赭曲霉毒素A现场快速检测之用。

关键词: 赭曲霉毒素A, 胶体金, 试纸条

Abstract: A method for rapid detection of Ochratoxin A by colloidal gold strip was introduced. Four steps were involved in the process of the preparation of the strip, including production of colloidal gold, manufacture of immunogold, installation and analysis of the strip. With the strip, 10ng/ml of Ochratoxin A was detected in less than 10 min. Apart from very short time to get test results, the strip possessed benefits of user-friendly format and relatively inexpensive to make. So it is ideally suited for on site testing of Ochratoxin A.

Key words: Ochratoxin A, colloidal gold, strip