食品科学 ›› 2007, Vol. 28 ›› Issue (10): 41-45.

• 基础研究 • 上一篇    下一篇

乌骨鸡活性肽组成成分及体外抗氧化活性研究

 林霖, 田颖刚, 谢明勇, 吴光杰, 孙亚真   

  1. 南昌大学食品科学与技术国家重点实验室; 南昌大学食品科学与技术国家重点实验室 江西南昌330047; 江西南昌330047;
  • 出版日期:2007-10-15 发布日期:2011-11-22

Research on Antioxidant Activity in vitro and Molecular Weight Distribution of Black-bone Silky Fowl Bioactive Peptides

 LIN  Lin, TIAN  Ying-Gang, XIE  Ming-Yong, WU  Guang-Jie, SUN  Ya-Zhen   

  1. State Key Laboratory of Food Science and Technology, Nanchang University; Nanchang 330047, China
  • Online:2007-10-15 Published:2011-11-22

摘要: 本实验对乌骨鸡活性肽的蛋白质含量、多肽含量、游离氨基酸含量和多肽的分子量分布进行了研究,并通过测定乌骨鸡活性肽对Fenton反应产生的羟自由基、邻苯三酚自氧化产生的超氧阴离子的清除率,考察乌骨鸡活性肽的体外抗氧化能力。乌骨鸡活性肽组成分析表明:活性肽中蛋白质含量为90.53%,其中主要成分为小分子多肽(51.46%)和氨基酸(36.17%)。乌骨鸡活性肽分子量分布的凝胶色谱法测定结果显示其分子量集中在1900Da以下,其中小肽所占相对比例较大。体外抗氧化实验结果表明:乌骨鸡活性肽具有较强的清除羟自由基的能力,其清除率与浓度呈剂量关系;并能明显降低邻苯三酚自氧化速率,抑制率与浓度呈正相关,但与肌肽比较,乌骨鸡活性肽的体外抗氧化能力相对要弱。

关键词: 乌骨鸡, 活性多肽, 分子量分布, 氨基酸, 抗氧化活性

Abstract: The content of protein, polypeptides, free amino acids and distribution of molecular polypeptide weight of in BSF bioactive peptides were analyzed in this research. And the anti-oxidant activities of black bone silky fowl (BSF) bioactive peptides were evaluated by determination of the scavenging activities against hydroxyl radicals and superoxide radicals produced by Fenton reaction and pyrogallol autoxidation respectively. The results showed the protein content was 90.53% in BSF bioactive peptide, in which polypeptide amounted for 50.46% and amino acids 36.17%. The major molecular weight of BSF bioactive peptides was less than 1900 Da and small weighted peptide was dominated. BSF Bioactive peptides had a strong scavenging effect against hydroxyl radicals, and the scavenging activity showed a positive dose-response relationship with BSF bioactive peptides concentration; Also, BSF Bioactive peptide can decrease the speed of pyrogallol autoxidation, and the reducing effect showed a positive relationship with concentration, however its anti-oxidant activity in vitro is weaker than carnosine did.

Key words: black-bone silky fowl, bioactive peptides, antioxidant activity, molecular weight distribution, amino acids