食品科学 ›› 2005, Vol. 26 ›› Issue (6): 215-217.

• 分析检测 • 上一篇    下一篇

SFC和GC/MS快速测定茶叶中咖啡因(英文)

 郭亚东, 丁颖洁   

  1. 昆明医学院药学院
  • 出版日期:2005-06-15 发布日期:2011-09-19

Rapid Determination of Caffeine in Tea by SFC and GC/MS

 GUO  Ya-Dong, DING  Ying-Jie   

  1. Faculty of Pharmacy, Kunming Medical College
  • Online:2005-06-15 Published:2011-09-19

摘要: 采用超临界流体色谱和气-质联用分别测定茶叶中咖啡因,结果表明:(1)用CO2作流动相,加5%的CH3OH做改性剂,SFC所得结果令人满意,3min完成分析。(2)气-质联用测定7min完成。两个方法都可用于茶叶中咖啡因的快速分析,茶叶中共存的其它组分不干扰测定。

关键词: 超临界流体色谱, 气-质联用, 茶叶, 咖啡因

Abstract: Chromatography (SFC) and GC/MS have been developed in this paper. The data showed (1) using a mobile phase of carbon dioxide modified with 5% methanol ,SFC achieved excellent result, and the time for the analysis was less than 3 minutes; (2) using GC/MS, the analysis was finished within 7 minutes. The two methods could be used to determine caffeine in tea rapidly. The other components contained in tea did not interfere the assay.

Key words: SFC (Supercritical Fluid Chromatography), GC/MS, tea, caffeine