食品科学 ›› 2005, Vol. 26 ›› Issue (6): 208-209.

• 分析检测 • 上一篇    下一篇

卵磷脂保健食品中磷脂酰胆碱的含量测定

 王永平, 谭永霞, 肖飞   

  1. 湖北省中药现代化工程技术研究中心
  • 出版日期:2005-06-15 发布日期:2011-09-19

The Quanlity Dedermintion of PC in Lethicin Function Foods

 WANG  Yong-Ping, TAN  Yong-Xia, XIAO  Fei   

  1. Hubei Engingeering Technology Centre of Mordernization of TCM
  • Online:2005-06-15 Published:2011-09-19

摘要: 建立一种准确、快速的对卵磷脂保健食品中的主要组分磷脂酰胆碱(PC)进行定量分析的方法。采用紫外分光光度法,将卵磷脂的正己烷提取液与0.5%碘-正己烷溶液反应后,于292nm波长处测定吸光度。吸光度与磷脂酰胆碱的含量呈线性相关,吸光值在0.35~1.1范围内线性关系良好,相关系数为0.9994。本方法准确、快速,简便,适于卵磷脂保健食品中磷脂酰胆碱的含量测定。

关键词: 卵磷脂, 磷脂酰胆碱, 紫外分光光度法, 含量测定

Abstract: To develop an accurate、rapid method to determine the quantity of the main ingrediant PC in lethicin function foods. Extracting lethicin with hexane,then adding 0.5%iodide-hexane and reacting,absorbance was detected by UV spectrophotometry at a wavelength 292nm. The absorbance calibration curve of PC was in good linearity over the range of 0.35~1.1, r=0.9994(n=5). The method is accurate,rapid simple and suitable to determine the quantity of PC in lethicin function foods.

Key words: Lethicin, PC, UV spectrophotometry, quantitive determination