关键词: 桑叶, 黄酮, 多糖, 正交实验, 大孔树脂, 分离工艺

Abstract: Object: To optimize the extracting and separating process for flavonoids and polysaccharides from mulberry leaves. Methods: Pass the single factor experiment to get the factors that show the important influence and by orthogonal design to the factors to optimize the extracting and separating process. Results: The optimum extracting and separating conditions were 1:30 the solid to liquid, 80℃ extracting temperature and pH=8 extracting and separating process for flavonoids, and were 1:40 the solid to liquid, 70℃ extracting temperature and pH=7 extracting and separating process for polysaccharides from mulberry leaves. Conclusion: The optimized process is stable and highly efficient with the water to extract and separate with macroeticular resin flavonoids and polysaccharides from mulberry leaves.

Key words:  mulberry leaves, flavonoids, polysaccharids, orthogonal design, macroeticular resin, the extracting and separating process