食品科学 ›› 2005, Vol. 26 ›› Issue (6): 39-43.

• 基础研究 • 上一篇    下一篇

提取条件对白鲢肌动球蛋白得率及Ca~(2+)-ATPase活性的影响

 刘茹, 刘海梅, 熊善柏, 严菁   

  1. 华中农业大学食品科技学院
  • 出版日期:2005-06-15 发布日期:2011-09-19

Effect of Extraction Condition on the Recovery Rate and Ca~(2+)-ATPase Activity of Actomyosin in Silver Carp

 LIU  Ru, LIU  Hai-Mei, XIONG  Shan-Bai, YAN  Jing   

  1. College of Food Science and Technology, Huazhong Agricultural University
  • Online:2005-06-15 Published:2011-09-19

摘要: 本文以白鲢为原料,研究肌动球蛋白提取条件对其得率及Ca2+-ATPase活性的影响。结果表明:用水漂洗1次,低盐磷酸缓冲液清洗3次后肌动球蛋白得率及其Ca2+-ATPase活性较高;肌动球蛋白得率及其Ca2+-ATPase活性均随浸提温度的升高而下降,温度低于20℃时对Ca2+-ATPase活性的影响较小,但高于20℃时,Ca2+-ATPase活性随浸提温度的升高而迅速下降;浸提时间极显著地影响Ca2+-ATPase活性,离心速度对得率影响显著,其最佳浸提时间、离心速度分别为22h和8000r/min。在所得的适宜提取工艺条件下,肌动球蛋白得率为12.57%,Ca2+-ATPase活性可达60.62U/g。

关键词: 白鲢, 肌动球蛋白, 提取, Ca2+-ATPase活性

Abstract: Effect of extraction condition on the recovery rate and Ca 2+ -ATPase activity of actomyosin was investigated. The result suggested that the recovery rate and Ca 2+ -ATPase activity were high respectively after water-washing one time and buffer- washing three times. Both of them decreased with increasing extraction temperature. The activity hardly changed below 20℃, but it obviously went down while extraction temperature was over 20℃. Extraction time had significant influence on the activity, and also centrifugal velocity on the recovery rate. And the optimal time was 20 hours and centrifugal velocity 8000r/ min, respectively. Under the extraction condition that had been optimal, the recovery rate of actomyosin was 12.57% and Ca 2+ - ATPase activity reached 60.62U/g.

Key words: silver carp, actomyosin, extraction, Ca2+-ATPase activity