食品科学 ›› 2007, Vol. 28 ›› Issue (10): 200-203.

• 工艺技术 • 上一篇    下一篇

响应曲面法优化微波辅助提取黑灵芝孢子多糖工艺研究

 黄璞, 谢明勇, 聂少平, 陈奕, 李昌, 谢建华   

  1. 南昌大学食品科学与技术国家重点实验室; 南昌大学食品科学与技术国家重点实验室 江西南昌330047; 江西南昌330047;
  • 出版日期:2007-10-15 发布日期:2011-11-22

Study on Microwave-assisted Extraction of Polysaccharides from Spores of Ganoderma atrum with Response Surface Analysis

 HUANG  Pu, XIE  Ming-Yong, NIE  Shao-Ping, CHEN  Yi, LI  Chang, XIE  Jian-Hua   

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
  • Online:2007-10-15 Published:2011-11-22

摘要: 为优化黑灵芝孢子粉多糖的微波提取工艺,在单因素试验基础上,选择提取温度、提取时间以及水料比为自变量,多糖提取率为响应值,采用中心组合设计的方法,研究各自变量及其交互作用对多糖提取率的影响。利用SAS和响应面分析相结合的方法,模拟得到二次多项式回归方程的预测模型,并确定微波提取多糖最佳条件为:温度129℃、时间27min、水料比为31:1。在此条件下,多糖提取率达到2.64%。

关键词: 黑灵芝孢子粉, 微波辅助提取, 多糖, 响应面分析

Abstract: To optimize the microwave-assisted extraction(MAE) of polysaccharide from spores of Ganoderma atrum(PSGA), on the base of the single factor investigation, the extraction temperature, extraction duration and the ratio of water to material were studied with the central composite design and their interactions on the yield of polysaccharide from spores of Ganoderma atrum were also investigated. The predictive polynomial quadratic equations model was developed by SAS software. Optimal conditions of MAE of polysaccharides from spores of Ganoderma atrum can be concluded as follows: 27 min at 129 ℃, the ratio of solvent to material 31:1, purified water as the solvent. On these conditions, the yield of the PSGA is up to 2.64%.

Key words: spores of Ganoderma atrum, microwave-assisted extraction, polysaccharides, response surface analysis