食品科学 ›› 2005, Vol. 26 ›› Issue (7): 198-199.

• 分析检测 • 上一篇    下一篇

RP-HPLC法测定茯苓中去氢乙酰茯苓酸的含量

 易中宏, 胥秀英, 郑一敏, 王龙   

  1. 重庆大学化学化工学院,重庆工学院生物工程学院
  • 出版日期:2005-07-15 发布日期:2011-09-19

Quantitative Determination of Dehydropachymic Acid in Poria cocos by RP-HPLC

 YI  Zhong-Hong, XU  Xiu-Ying, ZHENG  Yi-Min, WANG  Long   

  1. 1.College of Chemistry and Chemical Engineering, Chongqing University;2.College of Bioengineer, Chongqing Institute of Technology
  • Online:2005-07-15 Published:2011-09-19

摘要: 目的:建立RP-HPLC法测定茯苓中去氢乙酰茯苓酸的含量。方法:采用Phenomenex LunaC18柱(4.6mm×150mm,5μm),以乙腈:0.05%H3PO4水(64:36,V/V)为流动相,流速1.0ml/min,检测波长242nm,柱温25℃,用外标法定量,测定茯苓中去氢乙酰茯苓酸的含量。结果与结论:去氢乙酰茯苓酸的线性范围0.35~1.75μg,相关系数r=0.9991,回收率97.86%,RSD1.52%,该方法简便,快速,线性关系良好。

关键词: 去氢乙酰茯苓酸, 茯苓, 高效液相色谱法

Abstract: Objective: To determine the content of dehydropachymic acid in Poria cocos. Methods: The RP-HPLC methodwas used to determine the content of dehydropachymic acid in Poria cocos. The analysis was carried out on Phenomenex LunaC18 column(4.6mm×150mm,5μm). The mobile phase was CH3CN-0.05%H3PO4 (64:36, V/V), flow-rate 1.0ml/min, wavelength242nm and temperature 25℃. Results and conclusion: The method was simple and had a good linear relationship. The liner rangewas 0.35~1.75μg and gave a correlation (r) of 0.9991. The recovery was 97.86% and RSD 1.52%. The method was easy todetermine the content of dehydropachymic acid in Poria cocos.

Key words: dehydropachymic acid, Poria cocos, HPLC