食品科学 ›› 2005, Vol. 26 ›› Issue (7): 78-81.

• 基础研究 • 上一篇    下一篇

荸荠英抑菌成分活性跟踪方法的研究

 刘欣, 郝淑贤, 赵力超, 黄慧, 陈永泉   

  1. 华南农业大学食品学院,南海水产研究所营养与食品工程研究室
  • 出版日期:2005-07-15 发布日期:2011-09-19

Study on Tracking Bacteriostatic Components in Puchiin

 LIU  Xin, HAO  Shu-Xian, ZHAO  Li-Chao, HUANG  Hui, CHEN  Yong-Quan   

  1. 1.College of Food South China Agricultural University;2.Nutrition and FoodEngineering Lab, South China Sea Fisheries Institute
  • Online:2005-07-15 Published:2011-09-19

摘要: 本文通过抑菌活性分析,确定敏感菌,运用硅胶板层析技术,研究生物自显影对荸荠英提取物抑菌成分的活性跟踪方法:用200mg/ml荸荠英提取物丙酮溶液点样4μl,以二元溶剂氯仿:甲醇=8.5:1.5展开,贴板10min,37℃培养10h即可得到清晰的抑菌斑色谱图。应用该方法可将荸荠英粗提物乙酸乙酯部分分离得到六个活性组分。

关键词: 荸荠英, 抑菌, 生物自显影, 分离度

Abstract: The biological development technique was used to provide a qualitative analysis method for tracking bacferiostaficcomponents in puchiin. In the study, the Bacillus subtilis was regarded as indicator organism. The optimum conditions wereobtained as follows with 200mg/ml acetone solution extract: the volumn of sample was 4μl, the diffusion time and the culturetime were 10min and 10h respectively under the temperature of 37℃. Six different antibacterial points appeared with themixture of chloroform and carbinol in the ratio of 8.5 to 1.5.

Key words: puchiin, bacteriostatic, bioautography, Rf