食品科学 ›› 2007, Vol. 28 ›› Issue (10): 258-260.

• 工艺技术 • 上一篇    下一篇

大孔吸附树脂分离纯化高良姜黄酮类化合物的研究

 侯红瑞, 陈玲, 冯毅凡, 张志平   

  1. 华南理工大学轻工与食品学院; 广东药学院药物研究所; 华南理工大学轻工与食品学院 广东广州510640; 广东广州510640; 广东广州510224;
  • 出版日期:2007-10-15 发布日期:2011-11-22

Study on Separation and Purification of Flavonoids in Alpinia officinarum Hance by Macroporous Adsorption Resin

 HOU  Hong-Rui, CHEN  Ling, FENG  Yi-Fan, ZHANG  Zhi-Ping   

  1. 1.College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China; 2.Institute of Drug Research, Guangdong College of Pharmacy, Guangzhou 510224, China
  • Online:2007-10-15 Published:2011-11-22

摘要: 利用大孔吸附树脂柱层析法对高良姜黄酮粗提物进行纯化研究,以紫外分光光度法检测纯化后黄酮纯度和得率,作为纯化效果的评价。并对各种可能影响黄酮粗提物纯化的因素进行研究,得出较佳的纯化工艺参数,经纯化后的高良姜黄酮的含量可达到90.32%,得率为49.91%。

关键词: 高良姜, 黄酮, 大孔吸附树脂, 纯化

Abstract: In this experiment column chromatography composed of the macroporous adsorption resin was used to purify the extract of flavonoids in Alpinia officinarum Hance. The content of flavonoids in the sample and the yield, as an evaluation of the result of purification, was determined in UV spectrophotometer. The optimum technological parameters of purification were obtained, which included all the factors of affecting purification of flavonoids. The results showed the content of flavonoids in the sample was up to 90.32%, and the yield was 49.91% by means of purifications via macroporous adsorptionresin from Alpinia officinarum Hance.

Key words: Alpinia officinarum Hance, flavonoid, macroporous, adsorption resin, purification