食品科学 ›› 2007, Vol. 28 ›› Issue (10): 267-271.

• 工艺技术 • 上一篇    下一篇

发酵菜籽粕脱毒工艺优化研究

 陆豫, 余勃, 藏超, 杨胜远, 邓丽娟   

  1. 南昌大学中德联合研究院食品科学与技术国家重点实验室 南昌市生物化工重点实验室; 南昌大学中德联合研究院食品科学与技术国家重点实验室; 南昌市生物化工重点实验室; 广西大学生命科学与技术学院; 江西南昌330047; 广西南宁530004;
  • 出版日期:2007-10-15 发布日期:2011-11-22

Optimization of Culture Medium of Multiple Strains for Reduction of Toxicity in Rapeseed Meals

 LU  Yu, YU  Bo, CANG  Chao, YANG  Sheng-Yuan, DENG  Li-Juan   

  1. 1.Sino-German Joint Research Institute, State Key Laboratory of Food Science and Technology, Nanchang Key Laboratory of Bioehemical Engineering, Nanchang University, Nanchang 330047, China; 2.College of Life Science and Technology, Guangxi University, Nanning 530004, China
  • Online:2007-10-15 Published:2011-11-22

摘要: 目的:探索菜籽粕固体混菌发酵脱毒的最佳工艺条件。方法:采用中心组合旋转实验设计,评价发酵工艺中的5个关键因素,即初始pH、接种量、发酵温度、发酵时间和水料比及其交互作用对硫甙脱除率的影响,确定最佳反应条件。结果:建立了5个发酵条件的一个2次多项式数学模型,该模型极显著(p<0.0001),拟合优度良好。借助模型方程产生的等高线图及统计软件,分析得出最大硫甙脱除率下各个因子的最适水平分别为pH5.1,接种量6.2%,水料比2:1,发酵时间47h,发酵温度29℃。在此条件下,硫甙脱除率达到了97%。结论:应用响应曲面法可适用于不同复配菌种的发酵工艺优化方法,科学有效的。

关键词: 菜籽粕, 脱毒, 固体发酵, 中心旋转组合设计

Abstract: Objects: To research the optimization of reduction of toxicity by fermentation of rapeseed meals. Methods: A central composite rotatable design with two star points according to the principles of response surface methodology was adopted. The influences of pH, time, temperature, seed content and the ration of water to material and their interactions on the reduction of toxicity were valuated and the optimum variable combination for the highest reduction ratio was determined with the assistance of statistical software and the contour plots. Results: A good quadratic polynomial model was obtained. According to the ANOVA, the model was significant (p<0.0001) and there was no lack of fit. By analyzing the contour p lots generated by the model, the optimal levels for each variable were as follow: pH 5.1, time 47 h, temperature 29 ℃, seed content 6.2% and the ratio of water to material 2:1. On these conditions, the response value was up to 97%. Conclusion: the above results demonstrated that, otimization of reduction of toxicity by fermentation of rapeseed meals using RSM was reasonable and efficient. The obtained model was workable in present tested range.

Key words: rapeseed meals, reduce toxicity, solid fermentation, central composite rotatable design