食品科学 ›› 2005, Vol. 26 ›› Issue (8): 156-158.

• 工艺技术 • 上一篇    下一篇

酶解与膜法联用制备蚕蛹生物功能活性肽

 张明春, 韩克勤, 张媛, 许陶, 张巾英   

  1. 天津商学院生物技术与食品科学学院制药工程系,天津大学药物科学与技术学院
  • 出版日期:2005-08-15 发布日期:2011-09-19

Preparation Biological Active Peptide from Fresh Silkworm Chrysalis with Method of Enzymolysis and Ultrafiltration

 ZHANG  Ming-Chun, HAN  Ke-Qin, ZHANG  Yuan, XU  Tao, ZHANG  Jin-Ying   

  1. 1.Department of Pharmacy Engineering, College of Bio-technology and Food Science, Tianjin University Commerce;2.College of Pharmaceuticals and Biotechnology, Tijin University
  • Online:2005-08-15 Published:2011-09-19

摘要: 目的:分离制备蚕蛹中的生物活性肽。方法:以蚕蛹为原料,经防褐变,除脂肪处理后,酶解与膜法联用,经胰蛋白酶解的蚕蛹蛋白水解液,通过截留分子量一万超滤膜,得到蚕蛹生物活性肽。培养酵母菌,测定蚕蛹生物活性肽对糖分解代谢的影响。结果:酶解与膜法联用分离制备的蚕蛹生物活性肽,对糖分解代谢丙酮酸生成有促进作用。

关键词: 蚕蛹, 生物功能活性肽, 酶解与膜法联用

Abstract: The work is preparation of biological active peptide from fresh silkworm chrysalis. After degreasing ,the protein of silkworm chrysalis was enzymolysis by trypsin. The enzymolysis condition is trypsin 0.5%, temperature 50℃, 5h. Biological active peptide be preparation from enzymolysis liquid with method of ultrafiltration. Using microorganism model as it makes, the biological active peptide be detected, which promote glycolysis.

Key words: silkworm chrysalis, biological active peptide, enzymolysis, ultrafiltration