食品科学 ›› 2005, Vol. 26 ›› Issue (8): 150-152.

• 工艺技术 • 上一篇    下一篇

杏鲍菇原生质体制备技术的研究

 陈敏, 蒋予箭, 姚善泾, 鲁毅, 朱林华   

  1. 浙江大学化学工程与生物工程系,浙江工商大学生物工程系
  • 出版日期:2005-08-15 发布日期:2011-09-19

Study on the Preparation of Pleurotus eryngii Protoplast

 CHEN  Min, JIANG  Yu-Jian, YAO  Shan-Jing, LU  Yi, ZHU  Lin-Hua   

  1. 1.Department of Chemical and Biological Engineering, Zhejiang University; 2.Department of Biotechnology Engineering, Zhejiang Gongshang University
  • Online:2005-08-15 Published:2011-09-19

摘要: 本文详细地探讨了杏鲍菇原生质体制备条件。结果表明:用0.6mol/L的甘露醇配制成质量比为2.5%,pH值为4.5~5的溶壁酶酶液,在酶液量与菌丝体的比值为20:1(V/W),温度30℃,转速为50r/min条件下酶解3h,可得到最高的原生质体得率。其原生质体数可达15.35×107个/ml。本研究为今后进行杏鲍菇原生质体的诱变及融合育种奠定基础。

关键词: 杏鲍菇, 菌丝体, 原生质体, 制备

Abstract: The conditions of preparation of Pleurotus eryngii protoplast were studied in details and systematically for the first time. Results showed that: the highest production rate of protoplast could be obtained by using 0.6mol/L mannitol, 2.5% lywallzyme and digesting for 3 h at the condition of 30℃, and 50r/min, pH value 4.5~5. The protoplast number of Pleurotus eryngii came to 15.35×107/ml.

Key words: Pleurotus eryngii, mycelial, protoplast, preparation