食品科学 ›› 2005, Vol. 26 ›› Issue (8): 146-149.
• 工艺技术 • 上一篇 下一篇
范丽娟, 潘道东
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FAN Li-Juan, PAN Dao-Dong
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摘要: 本实验采用硫酸铵一次性盐析、DEAE-SepharoseFF离子交换层析和SephacrylS-200凝胶过滤层析法,分离纯化免疫初乳中的IgG,并采用SDS-PAGE电泳、单向火箭免疫电泳及试管凝集法对IgG分离物进行了定性定量检测,结果表明所得IgG纯度高,活性大。
关键词: 免疫初乳, IgG, 分离纯化
Abstract: IgG precipitated by ammonium sulfate from Immune colostrum was isolated and purified by DEAE-Sepharose FF Ion exchange chromatography and Sephacryl S-200 Gel filtration chromatography. IgG was validated by SDS-PAGE electrophoresis, one-dimension rocket immunoelectrophoresis and the methods of tube agglutination. The results demonstrated that based on high activity, the purity of IgG was significant increased.
Key words: immune colostrums, IgG, isolation and purification
范丽娟, 潘道东. 免疫初乳中IgG的分离与纯化[J]. 食品科学, 2005, 26(8): 146-149.
FAN Li-Juan, PAN Dao-Dong. Isolation and Purification of IgG from Immune Colostrums[J]. FOOD SCIENCE, 2005, 26(8): 146-149.
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