食品科学 ›› 2005, Vol. 26 ›› Issue (8): 146-149.

• 工艺技术 • 上一篇    下一篇

免疫初乳中IgG的分离与纯化

 范丽娟, 潘道东   

  1. 南京师范大学生命科学学院,南京师范大学金陵女子学院食品科学与营养系
  • 出版日期:2005-08-15 发布日期:2011-09-19

Isolation and Purification of IgG from Immune Colostrums

 FAN  Li-Juan, PAN  Dao-Dong   

  1. 1.Life Science College, Nanjing Normal University;2.Food Science and Nutrition Department, Ginling Women’s College, Nanjing Normal University
  • Online:2005-08-15 Published:2011-09-19

摘要: 本实验采用硫酸铵一次性盐析、DEAE-SepharoseFF离子交换层析和SephacrylS-200凝胶过滤层析法,分离纯化免疫初乳中的IgG,并采用SDS-PAGE电泳、单向火箭免疫电泳及试管凝集法对IgG分离物进行了定性定量检测,结果表明所得IgG纯度高,活性大。

关键词: 免疫初乳, IgG, 分离纯化

Abstract: IgG precipitated by ammonium sulfate from Immune colostrum was isolated and purified by DEAE-Sepharose FF Ion exchange chromatography and Sephacryl S-200 Gel filtration chromatography. IgG was validated by SDS-PAGE electrophoresis, one-dimension rocket immunoelectrophoresis and the methods of tube agglutination. The results demonstrated that based on high activity, the purity of IgG was significant increased.

Key words: immune colostrums, IgG, isolation and purification