大杯伞菌丝体培养及其延缓离体腓肠肌疲劳的研究

食品科学 ›› 2005, Vol. 26 ›› Issue (8): 86-90.

大杯伞菌丝体培养及其延缓离体腓肠肌疲劳的研究

黄清荣, 辛晓林, 杨立红, 潘永胜

烟台师范学院生命科学学院

出版日期:2005-08-15 发布日期:2011-09-19

Study of the Submerged Culture of Clitocybe maxima Mycelia and Its Effect on Isolated Skeletal Muscu-lar Fatigue Induced by Electric Stimulation in Toad

HUANG Qing-Rong, XIN Xiao-Lin, YANG Li-Hong, PAN Yong-Sheng

College of Life Science, Yantai Normal University

Online:2005-08-15 Published:2011-09-19

摘要/Abstract

摘要： 目的:探讨大杯伞菌丝体培养的最佳液体培养基及其对骨骼肌疲劳的影响。方法:采用深层培养方法,筛选出较优的碳源、氮源、无机盐,设计三因素四水平的正交试验,筛选大杯伞的最适培养基;采用脉冲式电流直接刺激蟾蜍离体腓肠肌作为疲劳模型,观察大杯伞菌丝体和任氏液对蟾蜍腓肠肌收缩能力的影响。结果:最适培养基为红糖2%、麦麸3%、磷酸二氢钾0.2%;0.8、0.3mg/ml的大杯伞菌丝体能够延长肌肉收缩下降到最大收缩幅度的90%、50%和10%的时间,与对照组相比有统计学意义,0.1mg/ml的大杯伞菌丝体能够延长肌肉收缩幅度下降到最大收缩幅度的90%的时间,与对照组相比有统计学意义(p<0.05)。结论:大杯伞菌丝体对离体骨骼肌的疲劳有延缓作用。

关键词: 大杯伞, 深层培养, 正交试验, 离体腓肠肌, 肌肉疲劳

Abstract: Object: To study the submerged culture of Clitocybe maxima mycelia and its effect on skeletal muscle fatigue. Method: Selecting the optimum carbon sources, nitrogen sources and inorganic salt to get the optimum liquid medium recipe by the orthogonal test in three factors and four levers; Isolated toad gastrocnemius stimulated by pulse electricity was regarded as fatigue model and Clitocybe maxima mycelia was compared with Ringer solution to see what influence they had on muscle contract ability. Results: The optimum liquid medium recipe was brown sugar 2%, wheat-bran 3%, KH2PO4 0.2%. Clitocybe maxima mycelia of 0.8mg/ml and 0.3mg/ml could delay the time when muscle contract extent was 90%, 50% and 10% of its maximum, while that of 0.1mg/ml could delay the time when contract extent was 90% of its maximum(p<0.05). Conclusion: Clitocybe maxima mycelia had the function of delaying fatigue of isolated skeletal muscle.

Key words: Clitocybe maxima, submerged culture, orthogonal test, isolated gastrocnemius, muscle fatigue

黄清荣, 辛晓林, 杨立红, 潘永胜. 大杯伞菌丝体培养及其延缓离体腓肠肌疲劳的研究[J]. 食品科学, 2005, 26(8): 86-90.

HUANG Qing-Rong, XIN Xiao-Lin, YANG Li-Hong, PAN Yong-Sheng. Study of the Submerged Culture of Clitocybe maxima Mycelia and Its Effect on Isolated Skeletal Muscu-lar Fatigue Induced by Electric Stimulation in Toad[J]. FOOD SCIENCE, 2005, 26(8): 86-90.

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