食品科学 ›› 2005, Vol. 26 ›› Issue (8): 55-57.

• 基础研究 • 上一篇    下一篇

平菇水溶性多糖结构表征与体外抗氧化作用

 程超, 李伟, 汪兴平   

  1. 湖北民族学院生物科学与技术学院,西南农业大学园林学院
  • 出版日期:2005-08-15 发布日期:2011-09-19

Study on the Structure Analysis and Antioxidation Effect in vitro of Water Soluble Polysaccharide of Pleurotus ostreatus

 CHENG  Chao, LI  Wei, WANG  Xing-Ping   

  1. 1.Bioscience and Technology College, Hubei Nationalities Institute; 2. Horticulture and Forest School, Southwest Argriculture University
  • Online:2005-08-15 Published:2011-09-19

摘要: 本文主要采用酶法辅助热水提取平菇多糖,乙醇沉淀,并用正交实验确定最佳条件,Sevage法脱蛋白,乙醇分级沉淀后,得到A、B、C、D四个组分,其中C用紫外和红外扫描证明其与蛋白质以复合物形式存在。同时在体外设计超氧自由基、羟基自由基等体系,测定平菇粗多糖对他们的清除作用,实验结果表明,酶法处理水溶性多糖的提取率达到15.7%,平菇粗多糖对超氧阴离子自由基、羟基自由基具有清除作用。

关键词: 平菇, 水溶性多糖, 结构, 抗氧化

Abstract: In this paper, the polysaccharides of Pleurotus ostreatus were extracted by enzymy and hot-water, and precipitated with perpendicular experiments. The protein in polysaccharides was removed by sevage way. The polysaccarides were graded to A、B、C、D by ethnol. The results showed that the extracting rate of water soluble polysaccharides by enzyme was 15. 7%, and the polysaccharides had antioxidation effect on the• OH and O•2.

Key words: Pleurotrs ostreatus, water soluble polysaccharides, structure, antioxidation