野生元蘑多糖的分离鉴定及其清除氧自由基作用

食品科学 ›› 2007, Vol. 28 ›› Issue (11): 81-85.

野生元蘑多糖的分离鉴定及其清除氧自由基作用

杨立红, 刘林德, 姜华, 王晓洁, 邢伟, 蔡德华

鲁东大学生命科学学院；鲁东大学化学与材料学院；鲁东大学生命科学学院山东烟台264025；山东烟台264025；

出版日期:2007-11-15 发布日期:2011-11-22

Isolation and Identification of Saccharides from Hohenbuehelia serotina and Its Effect on Scavening Oxygen Free Radicals

YANG Li-Hong, LIU Lin-De, JIANG Hua, WANG Xiao-Jie, XING Wei, CAI De-Hua

1.Department of Biological Science and Technology, Ludong University, Yantai 264025, China; 2.Department of Chemistry and Materials, Ludong University, Yantai 264025, China

Online:2007-11-15 Published:2011-11-22

摘要/Abstract

摘要： 元蘑烘干后经热水提取,乙醇沉淀得粗多糖。粗多糖经Sevag法除蛋白后DEAE-纤维素(OH-)柱层析,分离得到两种多糖组份HsI和HsII,Sepharose CL-4B测得HsI和HsII分子量均为2.7725×104,红外光谱和NMR波普分析表明HsI和HsII均为含α和β两种构型的D-吡喃糖葡聚糖,其α和β两种构型比值HsI为2:1和HsII为1:1。单糖的连接方式为1-4连接和1-6连接,其比值HsI为1:1和HsII为2:1。邻苯三酚自氧化法对其清除氧自由基作用测定结果表明,元蘑多糖粗品和纯品HsI和HsII均具有清除氧自由基作用。

关键词: 野生元蘑, 多糖, 分离鉴定, 清除氧自由基

Abstract: The saccharides of Hohenbuehelia serotina were extracted by hot water and then was deposited by ethanol and the DEAE cellulose column chromatography was used on the polysaccharide purification after the protein was excluded with Sevag method. The result indicated that the polysaccharide was composed of HsI and HsII. The Sepharose CL-4B method showed that molecular weights of HsI and HsII were all 2.7725×104 when Infrared Ray(IR) and Magnetic Resonance(NMR)assays showed that HsI and HsII were all D-glucopyranose (containing two kinds of configuration,αandβ, which αtoβof HsI was 1:1 while αtoβof HsII was 2:1), and they were all linked by 1-4 linkage and 1-6 linkage which the ratio of HsI was 1:1 and the ratio of HsII was 2:1. The pyrogallic acid autooxidation method, according to its assays on scavening oxygen free radicals, indicated that both polysaccharides and the pure HsI and HsII have anti-oxidant properties.

Key words: Hohenbuehelia serotina, polysaccharide, isolation, oxygen-derived free radicals

杨立红, 刘林德, 姜华, 王晓洁, 邢伟, 蔡德华. 野生元蘑多糖的分离鉴定及其清除氧自由基作用[J]. 食品科学, 2007, 28(11): 81-85.

YANG Li-Hong, LIU Lin-De, JIANG Hua, WANG Xiao-Jie, XING Wei, CAI De-Hua. Isolation and Identification of Saccharides from Hohenbuehelia serotina and Its Effect on Scavening Oxygen Free Radicals[J]. FOOD SCIENCE, 2007, 28(11): 81-85.

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