食品科学 ›› 2007, Vol. 28 ›› Issue (11): 122-125.

• 基础研究 • 上一篇    下一篇

毛竹叶柄多糖体外抗氧化作用研究

 丁红秀, 高荫榆, 晁红娟, 夏冬华   

  1. 南昌大学食品科学与技术国家重点实验室; 南昌大学食品科学与技术国家重点实验室 江西南昌330047江西师范大学生命科学学院; 江西南昌330027; 江西南昌330047;
  • 出版日期:2007-11-15 发布日期:2011-11-22

Study on Antioxidative Effects in vitro of Polysaccharide from Moso Bamboo Leaves (PMBL)

 DING  Hong-Xiu, GAO  Yin-Yu, CHAO  Hong-Juan, XIA  Dong-Hua   

  1. 1.State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; 2.College of Life Science, Jiangxi Normal University, Nanchang 330027, China
  • Online:2007-11-15 Published:2011-11-22

摘要: 研究毛竹叶柄多糖(polysaccharide from moso bamboo leaves,简称PMBL)的体外抗氧化作用。以芦丁和VC为对照,测定PMBL对二苯代苦味酰自由基(DPPH)、超氧阴离子自由基(O2-·)和羟自由基(·OH)的清除能力,同时用硫代巴比妥酸(TBA)法测定其对亚油酸过氧化的抑制作用。结果表明,PMBL对O2-·和·OH均具有较强清除作用,其IC50分别为5.98mg/L和5.55mg/L,效果优于VC和芦丁;对DPPH(IC50=36.08mg/L)的清除能力略弱于VC和芦丁;TBA实验表明PMBL抗脂质过氧化效果不够理想。

关键词: 毛竹叶柄, 多糖, 体外抗氧化, DPPH法, TBA法

Abstract: The aim of this study is to investigate the antioxidant effect in vitro of polysaccharide from moso bamboo leaves (PMBL). The scavenging capacities of PMBL on 1,1-diphenyl 2-picrylhydrazyl, superoxide anion radicals and hydroxyl free radicals were assayed. Meanwhile, the anti-olipid peroxidation activity on linoleum acid was studied by trio barbituric acid (TBA) system. The experimental results indicated that PMBL has stronger effect of scavenging O2-·( half clearance rates was 5.98 mg/L) and ·OH (half clearance rates was 5.55 mg/L) than VC and lutin, and the scavenging effect on DPPH is appreciably weaker than VC and lutin. From TBA experiment, it is proved that the antilipid peroxidation of linoleic acids was unideal.

Key words: moso bamboo leaves, polysaccharide, antioxidation in vitro, 2,2diphenyl-1-picryl-hydrazyl method, thiobarbituric acid chromatometry