食品科学 ›› 2007, Vol. 28 ›› Issue (11): 203-207.

• 工艺技术 • 上一篇    下一篇

从葡萄穗轴废渣中提取白藜芦醇的工艺研究

 康彦芳, 杨永杰, 封军来, 王璐琳, 伍丽娜   

  1. 天津渤海职业技术学院; 石家庄联合技术职业学院; 天津渤海职业技术学院 天津300402; 天津300402; 河北石家庄050228;
  • 出版日期:2007-11-15 发布日期:2011-11-22

Study on Extraction of Resveratrol from Waste Residue of Grape Spike-stalk

 KANG  Yan-Fang, YANG  Yong-Jie, FENG  Jun-Lai, WANG  Lu-Lin, WU  Li-Na   

  1. 1.Tianjin Bohai Vocational Technical College, Tianjin 300402, China; 2. Shijiazhuang Lionful Vocational College, Shijiazhuang 050228, China
  • Online:2007-11-15 Published:2011-11-22

摘要: 对从葡萄穗轴废渣中提取白藜芦醇的工艺进行了研究。用紫外分光光度计测定提取液吸光度,确定白藜芦醇的含量。考察了不同提取剂(甲醇、乙醇、丙酮、乙酸乙酯)、不同乙醇体积比、温度、时间、料液比以及提取次数对白藜芦醇提取率的影响,通过单因素试验和正交试验确定了最佳提取条件为:体积分数为50%乙醇做提取剂,提取温度70℃,料液比m(葡萄穗轴废渣):V(乙醇溶液)=1:15,提取时间4.0h,原料目数为60~100目,白藜芦醇一次提取率达0.34%。

关键词: 白藜芦醇, 葡萄穗轴, 提取, 紫外分光光度法

Abstract: The extraction of resveratrol from waste residue of grape spike-stalk was investigated. The yield of resveratrol was determined by ultraviolet spectrometry. The effect of different solvents such as methanol, ethanol, acetone and acetic ether on extraction yield was studied. Besides, the effects of volume fraction of ethanol, radio of solid/liquid,extraction temperature, extraction time, and extraxtion times on extraction yield of resveratrol were studied . On the basis of single factor and orthogonal test, the optimum condition were determined as follows: volume fraction of 50% ethanol solution as extractive solvent, extraction temperature 70 ℃, radio of solid/liquid 1:15, extraction time 4.0h, the mesh of material 60~100 mesh, the extraction yield of resveratrol could reach 0.34% one time.

Key words: resveratrol, grape spike-stalk, extraction, ultraviolet spectrometry