甘薯叶中绿原酸最佳提取工艺的研究

食品科学 ›› 2007, Vol. 28 ›› Issue (11): 245-248.

甘薯叶中绿原酸最佳提取工艺的研究

李文芳, 晏丽, 田春莲, 古鑫, 肖玉梅

吉首大学城乡资源与规划学院；吉首大学城乡资源与规划学院湖南张家界427000；湖南张家界427000；

出版日期:2007-11-15 发布日期:2011-11-22

Study on Optimum Extraction Process of Chlorogenic Acid in Sweet Potato Leaves

LI Wen-Fang, YAN Li, TIAN Chun-Lian, GU Xin, XIAO Yu-Mei

College of Resources and Planning Sciences, Jishou University, Zhangjiajie 427000, China

Online:2007-11-15 Published:2011-11-22

摘要/Abstract

摘要： 目的:优选甘薯叶中绿原酸提取的工艺条件。方法:用正交设计对传统水煎法、乙醇回流法、超声波法等提取工艺进行比较,以提取物中绿原酸的含量与提取率为评判指标,考察浓度、时间、料液比等因素对有效成分绿原酸提取率的影响。采用紫外分光光度法测定该成分的含量。结果:最佳提取工艺为乙醇回流法,最佳提取条件为:60%乙醇,料液比1:20,回流提取1.5h。此条件下绿原酸含量与提取率最高。影响提取的主次因素为:乙醇浓度>回流时间>料液比。

关键词: 甘薯叶, 绿原酸, 乙醇回流, 提取工艺

Abstract: Objective: To optimize the extraction process of chlorogenic acid in sweet potato leaves. Methods: With the content of the extract and extraction rate of chlorogenic acid as indexes by orthogonal design, several extraction processes were compared respectively, such as traditional decocting method, the ethanol refluxing method, the ultrasonic method etc. The factors affecting the extraction rate such as the concentration of extracting solvent, the extracting time, the ratio of solid and liquid were investigated. The content of chlorogenic acid was determine by ultraviolet-spectrophotometry. Results: The optimum extrac- tion is refluxing method. The optimum extraction conditions were: 75% ethanol, solid and liquid ratio 1:20 and refluxing 1.5 h. Under these conditions the content and chlorogenic acid and extraction rate reached the highest levels. The factors influencing this extraction efficiency were as follows: Consistency of ethanol>times of reflux>ratio of solid and liquid.

Key words: sweet potato leaves, chlorogenic acid, ethanol refluxing method, extraction process

李文芳, 晏丽, 田春莲, 古鑫, 肖玉梅. 甘薯叶中绿原酸最佳提取工艺的研究[J]. 食品科学, 2007, 28(11): 245-248.

LI Wen-Fang, YAN Li, TIAN Chun-Lian, GU Xin, XIAO Yu-Mei. Study on Optimum Extraction Process of Chlorogenic Acid in Sweet Potato Leaves[J]. FOOD SCIENCE, 2007, 28(11): 245-248.

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