关键词: 泡菜, 蛔虫卵, 鉴定, 荧光PCR, 食品, 寄生虫

Abstract: To identify the Ascaris egg- like materials from pickles, after the isolation of parasitic eggs from pickles, a real-time PCR assay for identifying Ascaris egg was set up and optimized. By comparison, the repeating treatment of the single egg in liquid nitrogen freezing with 37 ℃ thawing for 15 cycles is the best process other than 95 ℃ heating, and -86 ℃ freezing or 37 ℃ thawing in PCR buffer, or lysis with proteinase K digestion in the pre-treatment of the Ascaris egg. The subsequently developed real-time PCR detection is of much specificity and sensitivity for the Ascairs egg identification and can be used satisfactorily in the inspections ervice.

Key words: pickles, Ascaris egg, identification, real-time PCR(polymerase chain reaction), food, parasite