关键词: CaCl2, 肌原纤维骨架蛋白, 降解

Abstract: Myosin, actin, and myofibrils were incubated in 0, 100, 200, 300 mmol/L calcium chloride solutions respectively, and degradations were determined by SDS-PAGE and immunoblotting. Results showed that there were no obvious differences when myosin and actin were treated with 100, 200, 300 mmol/L concentration compared to 0 concentration.α-actinin and troponin-T were found that degradation occurred incubated in 100, 200, 300 mmol/L calcium chloride solutions, and degradation increased with concentration of calcium chloride increasing. Incubated in 300 mmol/L calcium chloride solutions, most of α-actinin degraded, and troponin-T degraded to smaller fragment less than 30 kD.

Key words: calcium chloride, myofibrillar skeletal proteins, degradation