食品科学 ›› 2007, Vol. 28 ›› Issue (12): 136-138.

• 工艺技术 • 上一篇    下一篇

羊栖菜多糖的提取及纯化

 蒋定文, 沈先荣, 贾福星, 陈伟, 刘李娜   

  1. 海军医学研究所; 海军医学研究所 上海200433; 上海200433;
  • 出版日期:2007-12-15 发布日期:2011-11-22

Extraction and Purification of Sargassum fusiforme Polysaccharides

 JIANG  Ding-Wen, SHEN  Xian-Rong, JIA  Fu-Xing, CHEN  Wei, LIU  Li-Na   

  1. Naval Medical Research Institute, Shanghai 200433, China
  • Online:2007-12-15 Published:2011-11-22

摘要: 目的:分离纯化羊栖菜多糖。方法:采用水煮醇沉法、酸煮醇沉法及乙醇脱脂后水煮醇沉三种方法提取羊栖菜粗多糖。利用Sevag法结合胰酶消化去蛋白,SephadexG200分离纯化多糖。结果:三种方法提取获得粗多糖含量分别为:24.35%、23.87%和17.87%。分子筛层析分离得到两个在A620和A280重合的组分,其硫酸根的含量分别为8.95%和3.92%。结论:从羊栖菜粗多糖分离得到两个含有硫酸基的蛋白聚糖。

关键词: 羊栖菜多糖, 分离, 纯化

Abstract: Objective: To study on the methods of extraction and purification polysaccharide from Sargassum fusiforme. Methods: Comparing three methods of extraction coarse polysaccharide from Sargassum fusiforme by hot water, acid hot water and hot water after degreased by ethanol. Crude polysaccharides were preliminarily purified with Sevag deprotein, and further purified by Sephadex G200 chromatography. Results: The 24.35%, 23.87% and 17.87% of crude polysaccharides were obtained from Sargassum fusiforme by these three methods, respectively. After purified, two types of protein polysaccharides were obtained from Sargassum fusiforme. The contents of their SO42-were 8.95% and 3.92%, respectively. Conclusion: Two protein polysaccharides containing sulfate were obtained from Sargassum fusiforme.

Key words: Sargassum fusiforme polysaccharides, extraction, purification