食品科学 ›› 2007, Vol. 28 ›› Issue (12): 248-250.

• 生物工程 • 上一篇    下一篇

HPLC-ESI-MS对珠蛋白酶解液中的血管紧张素I转换酶抑制肽的分离鉴定

 余奕珂, 胡建恩, 白雪芳, 杜昱光, 林炳承   

  1. 中国科学院大连化学物理研究所; 中国科学院大连化学物理研究所 辽宁大连116023中国科学院研究生院; 北京100049; 辽宁大连116023;
  • 出版日期:2007-12-15 发布日期:2011-11-22

Isolation and Identification of Angiotensin I-converting Enzyme Inhibitory Peptide Derived from Peptic Globin Hydrolysate by HPLC-ESI-MS

 YU  Yi-Ke, HU  Jian-恩, BAI  Xue-Fang, DU  Yu-Guang, LIN  Bing-Cheng   

  1. 1.Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; 2.Graduate University of Chinese Academy of Sciences, Beijing 100049, China
  • Online:2007-12-15 Published:2011-11-22

摘要: 本实验利用ESI-MS/MS对反相高效液相色谱分离的具有血管紧张素I转换酶(ACE)抑制活性的珠蛋白小肽的结构进行鉴定。结果表明:此肽的序列为Val-Val-Tyr-Pro-Trp-Thr(VVYPWT),位于猪的血红蛋白β链的34-39氨基酸序列片断,它对ACE有很好的抑制活性,其IC50为6.02μmol/L。

关键词: 反相高效液相色谱, 质谱, 珠蛋白, VVYPWT

Abstract: This paper described isolation and identification of angiotensin I-converting enzyme (ACE) inhibitory peptide derived from the peptic globin hydrolysate. After the isolation of ACE inhibitory peptide with reversed-phase high-perfor-mance liquid chromatography (RP-HPLC) on C18 column, one active fraction was obtained. The amino acid sequence was identified by electrospray ionization tandem mass spectrometry (ESI-MS-MS). The results showed that this peptide is Val-Val-Tyr-Pro-Trp-Thr(VVYPWT), corresponding to the 34-39 fragment of the β chain of porcine hemoglobin, with IC50 value as 6.02μmol/L.K e y w o r d s :RP-HPLC;MS;globin;VVYPWT

Key words: RP-HPLC, MS, globin, VVYPWT