食品科学 ›› 2005, Vol. 26 ›› Issue (9): 242-246.

• 工艺技术 • 上一篇    下一篇

表没食子儿茶素没食子酸酯(EGCG)的分离与制备

 王霞, 高丽娟, 林炳昌   

  1. 鞍山科技大学化工学院,鞍山科技大学分离技术中心
  • 出版日期:2005-09-15 发布日期:2011-09-20

Separation and Preparation of Epigallocatechin Gallate(EGCG)

 WANG  Xia, GAO  Li-Juan, LIN  Bing-Chang   

  1. 1.Shchool of Chemical Engineering , Anshan Science and Technology University; 2.Center of Separation Technology, Anshan Science and Technology University
  • Online:2005-09-15 Published:2011-09-20

摘要: 采用高效液相色谱技术,分离制备茶多酚中表没食子儿茶素没食子酸酯(EGCG)。制备工艺:茶多酚原料(EGCG,30%,HPLC)经乙酸乙酯萃取富集,得儿茶素粗提液(EGCG,60%,HPLC),粗提液经层析纯化得EGCG高纯度溶液,溶液经旋转蒸发浓缩,冷冻干燥,得白色粉末(EGCG,99%,HPLC)。

关键词: EGCG, 萃取, HPLC, 分离与制备

Abstract: Separation and preparation of Epigallocatechin Gallate(EGCG) from tea polyphenols was investigated by HPLC. The EGCG was enriched after green tea polyphenols(30%) was extracted by ethyl acetate. The purity of EGCG was 60%. EGCG was Separated and purified by HPLC. The high purification solution of EGCG was collected .The solution was evaporated and freeze-dried under vacuum. Finally, the product was abtained and its purity was more than 99%.

Key words: EGCG, extraction, high performance liquid chromatography, separation and preparation