食品科学 ›› 2005, Vol. 26 ›› Issue (9): 239-241.

• 工艺技术 • 上一篇    下一篇

野油菜黄单胞菌产胶XanA基因的分离

 刘莹   

  1. 上海水产大学食品学院
  • 出版日期:2005-09-15 发布日期:2011-09-20

The Separateness of Producing Colloid Gene XanA from Xanthomonas campestris

 LIU  Ying   

  1. College of Food Science, Shanghai Fisheries University
  • Online:2005-09-15 Published:2011-09-20

摘要: 本文主要建立了野油菜黄单胞菌产胶XanA基因的分离方法。通过基因工程手段,以黄原胶菌株基因组DNA为模板,优化PCR实验条件扩增得到XanA基因。最终以期使黄原胶的产量成倍成数倍地增长。本方法能快速简便的从野油菜黄单胞菌中分离出产胶基因XanA,且其含量较高,纯度较好,可用于文库和重组质粒的构建。

关键词: 野油菜黄单胞菌, 基因组DNA, 基因

Abstract: The separate method of producing colloid gene XanA from Xanthomonas campestris was promoted in this thesis. By the means of genetic engineering, the genetic DNA of Xanthan Gum was acted as template to obtain the gene XanA. And in the course of which, optimizing experiment of PCR analysis was made. Ultimately, the yield of Xanthan Gum is expected to increase geminately. This approach could be used to separate gene XanA rapidly. The purity of the obtained gene XanA was good enough for the establishment of genomic library and recombine plastid.

Key words: Xanthomonas campestris, genomic DNA, gene