食品科学 ›› 2005, Vol. 26 ›› Issue (9): 169-172.

• 基础研究 • 上一篇    下一篇

银耳粗多糖的纯化及抗氧化活性研究

 颜军, 徐光域, 郭晓强, 刘嵬, 李晓光, 苟小军   

  1. 成都大学中药化学实验室
  • 出版日期:2005-09-15 发布日期:2011-09-20

Research on the Purification and Anti-Oxidize Activity of Tremella Polysaccharide

 YAN  Jun, XU  Guang-Yu, GUO  Xiao-Qiang, LIU  Wei, LI  Xiao-Guang, GOU  Xiao-Jun   

  1. The Laboratory of Traditional Chinese Medicine of Chengdu university
  • Online:2005-09-15 Published:2011-09-20

摘要: 本文对银耳多糖进行提取、分离,并采用DEAE-纤维素和SephadexG-200进行纯化,得到均一酸性多糖TPP2。以银耳多糖清除羟基自由基能力为指标,对银耳多糖分离纯化的每一步进行跟踪,并研究了酸性多糖TPP2体外抗氧化活性。实验表明银耳酸性多糖TPP2清除羟自由基的作用明显,50%清除率的样品浓度为66.5μg/ml。银耳酸性多糖TPP2对猪油的过氧化有明显的抑制作用。

关键词: 银耳多糖, 纯化, 自由基清除, 抗氧化

Abstract: The tremella polysaccharide was purified by DEAE-Cellulose and Sephadex G-200, and then obtained a pure homopolysaccharide TPP2. The ability of the tremella polysaccharide to scavenge the hydroxyl free radical in the test was carefully observed, and the outside anti-oxidize activity of acidity polysaccharide was researched. It was proven in the test that TPP2 showed active ability in scavenging the hydroxyl free radical, the sample thickness was 66.5μg/ml in 50% scavenging rate.TPP2 restrains obviously the peroxide of lard.

Key words: tremella polysaccharide, purification, scavenging, anti-oxidize