食品科学 ›› 2005, Vol. 26 ›› Issue (9): 119-123.

• 基础研究 • 上一篇    下一篇

藠头中活性物质对白色念珠菌的抑制作用及其机理研究

 孟松, 胡胜标, 谢伟岸, 丁学知, 孙运军, 夏立秋   

  1. 湖南师范大学生命科学学院,湖南省微生物学与生物技术高校重点实验室
  • 出版日期:2005-09-15 发布日期:2011-09-20

Antifungal Effects and Mechanism of Bioactive Components of Allinm chinense on Candida albicans

 MENG  Song, HU  Sheng-Biao, XIE  Wei-An, DING  Xue-Zhi, SUN  Yun-Jun, XIA  Li-Qiu   

  1. College of Life Science, Hunan Normal University, Microbiology and Biotechnology Key Lab Hunan Province University
  • Online:2005-09-15 Published:2011-09-20

摘要: 藠头(Alliumchinense)活性物质对白色念珠菌(Candidaalbicans)的生长有较强的抑制作用,其活性物质洗脱液的最低抑菌浓度(MIC)值为0.2%(V/V);用藠头活性物质洗脱液处理白色念珠菌可导致细胞内OD260nm物质泄漏,且呈浓度和时间依赖性;藠头活性物质洗脱液可抑制白色念珠菌培养基的酸化,对细胞活性的影响也呈浓度和时间依赖性;通过红光图谱发现,藠头活性物质破坏了白色念珠菌细胞壁β-(1-3)-D葡聚糖的糖苷键而改变了细胞壁结构。

关键词: 藠头, 活性物质, 抗真菌, 细胞通透性, 培养基酸化, &beta, -(1-3)-D葡聚糖

Abstract: The bioactive components of Allium chinense inhibited multiplication of Candida albicans and its minimal inhibiting concentrations(MIC) was 0. 2%(V/V). The leakage of 260nm absorbing materials was increased in a dose-dependent manner after C.albicans treated by the bioactive components. The bioactive components inhibited glucose-induced acidification of the medium and increased Methylene Blue staining, also in a dose-dependent manner. The structure of β-(1-3)-D-glucan , which is a main composition of C.albicans cell wall, was changed by the bioactive components, analyzing by FT-Infrared spectrometer.

Key words: Allium chinense, bioactive components, antifungal, cell permeability, medium acidification, β-(1-3)-D-glucan