食品科学 ›› 2005, Vol. 26 ›› Issue (9): 100-103.

• 基础研究 • 上一篇    下一篇

壳聚糖/海藻酸钠微胶囊固定化磷脂酶A1及其结构考察

 孙万成, 蒋笃孝, 罗毅皓   

  1. 暨南大学食品科学与工程系,青海大学农牧学院
  • 出版日期:2005-09-15 发布日期:2011-09-20

Immobilized PLA1 by Chitosan/Alginate Microcapsule and Study on Its Structure

 SUN  Wan-Cheng, JIANG  Du-Xiao, LUO  Yi-Hao   

  1. 1.Department of Food Science and Engineering, Jinan University; 2.Faculty of the Agriculture and Veterinary, Qinghai University
  • Online:2005-09-15 Published:2011-09-20

摘要: 对壳聚糖/海藻酸钠微胶囊固定化磷脂酶A1方法进行优化,利用红外光谱和电子扫描显微镜(SEM),对微胶囊结构进行考察。表明微胶囊具有规则的通孔结构,有很大的内表面积,可增加包埋酶的效率,提高磷脂酶与卵磷脂的接触界面面积和固定化酶的活力回收。

关键词: 固定化磷脂酶A1, 微胶囊结构

Abstract: Chitosan, alginate, loaden enzyme have prominent effect for activity of immobilized enzyme by orthogonal experimental design, and getting optimum condition . IR spectra analyze chitosan/alginate microcapsule, and study on the inner structure of microcapsule by the ways of SEM which shows that it have regular micro-hole which have very large inner superficial area. Consequently, microcapsule can enhance activity of immobilized enzyme, increase interface area of reaction of lecithin and PLA1.

Key words: immobilized PLA1, microcapsule structure