食品科学 ›› 2005, Vol. 26 ›› Issue (12): 212-215.

• 营养卫生 • 上一篇    下一篇

不同苹果多酚提取物体外清除自由基及抗脂质过氧化的研究

 陈维军, 方琳, 戚向阳, 张俐勤, 杨尔宁, 谢笔钧   

  1. 华中农业大学食科学院,浙江医药高等专科学校实验室
  • 出版日期:2005-12-15 发布日期:2011-10-01

Research on Scavenging Effect on Radical and Inhibiting Effect on Lipid Peroxidation of Different Apple Polyphenol Extracts in vitro

 CHEN  Wei-Jun, FANG  Lin, QI  Xiang-Yang, ZHANG  Li-Qin, YANG  Er-Ning, XIE  Bi-Jun   

  1. 1.Department of Food Science, Huazhong Agricultural University; 2.Laboratory of Zhejiang Pharmaceutical College
  • Online:2005-12-15 Published:2011-10-01

摘要: 目的:研究了不同苹果多酚提取物清除羟基自由基、超氧阴离子自由基和脂质体过氧化产生的丙二醛的效果。方法:采用D-脱氧核糖法,试剂盒法及改良的TBA比色法分别测定苹果多酚提取物清除羟基自由基、超氧阴离子自由基和脂质体过氧化产生的丙二醛的效果。结果:在这三种抗氧化体系中,不同的苹果多酚提取物均有抗氧化效果,但其抗氧化效果因体系的不同而不同,以API,APII的抗氧化活性较强。结论:苹果多酚提取物具有抗氧化活性,其主要的抗氧化成分为分子量较小的酚类单体及原花青素低聚体。

关键词: 苹果多酚提取物, 清除, 自由基, 脂质过氧化

Abstract: Objective: To study the scavenging effects on hydroxyl radical (•OH), superoxidized anion radical (O2 •) and malondialdehyde (MDA) generated by lipidperoxidation of different apple polyphenol extracts. Methods: The DR spectro- photometry and improved TBA spectrophotometry and solvent box method were used to determined the scavenging effects on• OH, O•2and MDA. Results: Different apple polyphenol extracts all had antioxidant effects, but in different antioxidant systems, different apple polyphenol extracts had different antioxidant effects, and the antioxidant effects of AP I and AP II showed better. Conclusion: Apple polyphenol extracts had antioxidant activity. The main antioxidant components were phenols and proanthocyandins oligomer with low 
molecular weight.

Key words: apple polyphenol extract, scavenging effect, radical, lipid peroxidation