食品科学 ›› 2009, Vol. 30 ›› Issue (3 ): 243-248.doi: 10.7506/spkx1002-6630-200903055

• 生物工程 • 上一篇    下一篇

抗乙硫氨酸突变株产蛋氨酸发酵工艺条件的优化

林小秋,冯 琦,张东环,闵伟红*   

  1. 吉林农业大学食品科学与工程学院
  • 收稿日期:2008-10-29 出版日期:2009-02-01 发布日期:2010-12-29
  • 通讯作者: 闵伟红 E-mail:minwh2000@163.com
  • 基金资助:

    吉林省重大攻关项目(20065006)

Optimization of Fermentation Conditions for Methionine Production by Ethionine-resistant Mutant

LIN Xiao-qiu,FENG Qi,ZHANG Dong-huan,MIN Wei-hong*   

  1. (College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China)
  • Received:2008-10-29 Online:2009-02-01 Published:2010-12-29
  • Contact: MIN Wei-hong* E-mail:minwh2000@163.com

摘要:

本实验研究了抗乙硫氨酸突变株E31 营养条件和培养条件的优化,结果表明:突变株E31 发酵产蛋氨酸营养条件的最佳配方是葡萄糖11%、尿素0.8%、MgSO4·7H2O 0.08%、生物素3μg/100ml,最佳培养条件组合是发酵培养基pH6、发酵温度33℃、接种量8%、发酵时间84h,在此条件下蛋氨酸产量为2.086g/L,比优化前蛋氨酸产量(1.479 g/L)提高了0.607g/L。

关键词: 蛋氨酸, 抗乙硫氨酸突变株, 发酵

Abstract:

This study aimed to optimize the conditions of nutrition and cultivation of ethionine-resistant mutant E31. The results showed the best nutrition conditions are 11% glucose, 0.8% urea, 0.08% MgSO4·7H2O, 3 μg /100ml biotin, the best cultivation conditions are pH 6,fermentation temperature 33℃, inoculation size 8% and fermentation time 84 h, After the opimization, the yield of methionine is 2.086 g/L, 0.607g/L higher than that before the optimization.

Key words: methionine, ethionine resistant mutant, fermentation

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