食品科学 ›› 2007, Vol. 28 ›› Issue (12): 309-312.
• 生物工程 • 上一篇 下一篇
林剑, 孙莹, 刘晓艳, 徐世艾
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LIN Jian, SUN Ying, LIU Xiao-Yan, XU Shi-Ai
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摘要: 对琥珀酸产生菌S-1进行紫外线亚硝基胍的复合诱变后,筛选出琥珀酸产量高,遗传性状稳定的菌株S-57,并对其进行激光诱变,筛选出菌株SH-24,相同发酵条件下琥珀酸产量达到21.25g/L,相对于未诱变菌株产量提高了343倍。
关键词: 琥珀酸, 紫外线, 亚硝基胍, 激光, 诱变
Abstract: A high yield and stable succinic producing strain S-57 was obtained from the mutation of mucor S-1 treated with ultraviolet ray and nitrosoguanidine (NTG). After the strain S-57 was treated with He-Ne laser, a strain SH-24 was selected. The concentration of succinic acid is 21.25 mg/ml in the fermentation broth of the same conditions of fermentation or that is 343 times the strain before mutagenesis.
Key words: succinic acid, sltraviolet ray, nitrosoguanidine, laser, mutation
林剑, 孙莹, 刘晓艳, 徐世艾. 琥珀酸产生菌的诱变与选育[J]. 食品科学, 2007, 28(12): 309-312.
LIN Jian, SUN Ying, LIU Xiao-Yan, XU Shi-Ai. Mutagenesis and Screening of Succinic-producing Strain[J]. FOOD SCIENCE, 2007, 28(12): 309-312.
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