食品科学 ›› 2007, Vol. 28 ›› Issue (12): 309-312.

• 生物工程 • 上一篇    下一篇

琥珀酸产生菌的诱变与选育

 林剑, 孙莹, 刘晓艳, 徐世艾   

  1. 烟台大学化工制造省级重点实验室; 烟台大学化工制造省级重点实验室 山东烟台264005; 山东烟台264005;
  • 出版日期:2007-12-15 发布日期:2011-11-22

Mutagenesis and Screening of Succinic-producing Strain

 LIN  Jian, SUN  Ying, LIU  Xiao-Yan, XU  Shi-Ai   

  1. Provincial Key Laboratory of Chemical Industry Manufacture, Yantai University, Yantai 264005, China
  • Online:2007-12-15 Published:2011-11-22

摘要: 对琥珀酸产生菌S-1进行紫外线亚硝基胍的复合诱变后,筛选出琥珀酸产量高,遗传性状稳定的菌株S-57,并对其进行激光诱变,筛选出菌株SH-24,相同发酵条件下琥珀酸产量达到21.25g/L,相对于未诱变菌株产量提高了343倍。

关键词: 琥珀酸, 紫外线, 亚硝基胍, 激光, 诱变

Abstract: A high yield and stable succinic producing strain S-57 was obtained from the mutation of mucor S-1 treated with ultraviolet ray and nitrosoguanidine (NTG). After the strain S-57 was treated with He-Ne laser, a strain SH-24 was selected. The concentration of succinic acid is 21.25 mg/ml in the fermentation broth of the same conditions of fermentation or that is 343 times the strain before mutagenesis.

Key words: succinic acid, sltraviolet ray, nitrosoguanidine, laser, mutation