食品科学 ›› 2005, Vol. 26 ›› Issue (12): 160-164.

• 工艺技术 • 上一篇    下一篇

果胶酶高产菌株的激光选育及发酵条件的优化

 朱宏莉, 宋纪蓉, 张嘉, 黄洁, 徐抗震   

  1. 西北大学生命科学学院,西北大学化工学院
  • 出版日期:2005-12-15 发布日期:2011-10-01

The Breeding of High-yield Pectinase Producing Baterial Using He-Ne Laser and Studies on Its Optimal Fermentation Conditions

 ZHU  Hong-Li, SONG  Ji-Rong, ZHANG  Jia, HUANG  Jie, XU  Kang-Zhen   

  1. 1.Institute of Life Science, Northwest University; 2.Institute of Chemistry Engineering, Northwest University
  • Online:2005-12-15 Published:2011-10-01

摘要: 利用氦氖激光诱变原生质体筛选到了一株产果胶酶性能稳定且酶活明显提高的突变株ZH-2。其最适发酵条件为:以2%桔皮粉+1%乳糖作碳源,以1%(NH4)2SO4+0.3%酵母膏作氮源,在起始pH7.0,33℃摇床发酵32h左右达产酶高峰。分析ZH-2所产果胶酶的性质得出,酶作用最适条件为:pH6.5,50℃,以聚半乳糖醛酸为底物,酶的热稳定性实验显示,50℃保温60min后,酶活力基本不变。

关键词: 果胶酶, He-Ne激光, 诱变, 发酵条件, 原生质体

Abstract: A mutant strain ZH-2 with high yield of pectinase was selected by He-Ne laser mutagenesis on protoplasts of the original one. The optimal fermentation conditions were investigated: initial pH7.0, temperature 33℃, with 2% citrus peel powder and 1% lactose as carbon source, 1% (NH4)2SO4 and 0.3% yeast extract paste as nitrogen source, after fermenting for 32h on a rotary shaker. By preliminary analysis of the pectinase produced by ZH-2, optimum conditions for enzyme catalysis were pH6.5 at 50℃. It was stable and even retained 90% activity after incubation at 50℃ for 60min.

Key words: pectinase, He-Ne laser, mutagenesis, fermentation conditions, protoplasts