食品科学 ›› 2005, Vol. 26 ›› Issue (12): 115-118.

• 基础研究 • 上一篇    下一篇

Plackett-Burman设计在灵芝生长及产胞外多糖主要影响因子筛选中的应用

 陈志杰, 韩永斌, 沈昌, 陈德明, 顾振新   

  1. 南京农业大学农业部农畜产品加工与质量控制重点开放实验室,南京农业大学农业部农畜产品加工与质量控制重点开放实验室
  • 出版日期:2005-12-15 发布日期:2011-10-01

Application of Placket-Burman Design for Determining Key Factors on Mycelium Growth and Extracellular Polysaccharides Excretion of Ganoderma lucidum

 CHEN  Zhi-Jie, HAN  Yong-Bin, SHEN  Chang, CHEN  De-Ming, GU  Zhen-Xin   

  1. Key Laboratory of Food Processing and Quality Control, Ministry of Agriculture, Nanjing Agricultural University
  • Online:2005-12-15 Published:2011-10-01

摘要: 本文采用Plackett-Burman试验设计,选取葡萄糖、蔗糖、玉米浆干粉、蛋白胨、黄豆芽汁、KH2PO4、Na2HPO4、MgSO4、ZnSO4•7H2O、维生素B1、谷氨酸钠11个对灵芝生长及产胞外多糖相关因子进行筛选。统计分析表明:蔗糖(p=0.0119)、磷酸二氢钾(p=0.0111)和硫酸锌(p=0.0024)对灵芝菌丝生长具有显著影响;蔗糖(p=0.0165)、磷酸二氢钾(p=0.0103)和硫酸锌(p=0.0019)对灵芝产胞外多糖具有显著影响。

关键词: Plackett-Burman设计, 灵芝, 多糖, 深层发酵

Abstract: The Plackett-Burnam design was applied to screen out the key factors from the following eleven components, namely, glucose, sucrose, corn syrup powder, peptone, soybean bud juice, KH2PO4, Na2HPO4, MgSO4, ZnSO4•7H2O, VB1 and sodium glutamate, which would affect the mycelium growth and extracellular polysaccharides excretion of Ganoderma lucidum. With JMP software(version 4.0.5, SAS Institute Inc, USA)statistical analysis was performed. It was found that sucrose (p=0.0119), KH2PO4 (p=0.0111) and ZnSO4 (p=0.0024) were significant factors for mycelium growth; sucrose(p=0.0165), KH2PO4 (p=0.0103) and ZnSO4 (p=0.0019) were significant factors for extracellular polysaccharides excretion of Ganoderma lucidum.

Key words: Plackett-Burman design, Ganoderma lucidum, polysaccharide, submerged fermentation