食品科学 ›› 2006, Vol. 27 ›› Issue (7): 173-176.

• 工艺技术 • 上一篇    下一篇

牛舌菌液体发酵条件的优化研究

 陈文强, 邓百万, 李新生, 冯航   

  1. 陕西理工学院陕西省资源生物重点实验室; 西安文理学院生命科学系
  • 出版日期:2006-07-15 发布日期:2011-09-29

Study on Liquid Fermentative Conditions of Fistulina hepatica

CHEN  Wen-Qiang, DENG  Bai-Wan, LI  Xin-Sheng, FENG  Hang   

  1. 1.Shaanxi Key Laboratory of Bio-Resources, Shaanxi University of Technology, Hanzhong 723001, China; 2.Department of Life Science, Xi′an University of Arts and Science, Xi′an 710065, China
  • Online:2006-07-15 Published:2011-09-29

摘要: 采用摇瓶培养法对牛舌菌液体发酵的条件进行了研究。通过L9(34)正交试验确定的牛舌菌液体发酵的最适培养基组成为:玉米粉30.0g,黄豆粉7.0g,KH2PO42.0g,MgSO4·7H2O1.0g,VB10.01g;该菌株液体发酵的优化条件为:初始pH4.6~5.4,培养温度28℃,装液量60%,振荡速度210r/min,发酵时间6d,菌丝体生物量达14.7241g/50ml。

关键词:  , 牛舌菌, 液体发酵, 菌丝体

Abstract: This paper explore that suitable fermentative conditions, which affect the mycelia growth of Fistulina hepatica. Experimental results indicated that the optimum composition of a fermentative medium is as following:corn powder 30.0g, soya powder 7.0g, KH2PO4 2.0g, MgSO4·7H2O 1.0g, VB1 0.01g; pH4.6~5.4, the shaker rotation speed is at 210r/min, the temperature is at 28℃, the optimal culture media capacity is 60%. The highest yield of mycelia was obtained after fermentation for 6 days, the weight of mycelia reached 14.7241g/50ml fermentation liquid.

Key words: Fistulina hepatica, liquid fermentation, mycelia