食品科学 ›› 2006, Vol. 27 ›› Issue (7): 224-227.

• 分析检测 • 上一篇    下一篇

罗汉果中主要皂甙成分的高效液相分析研究

 张云竹, 戚向阳, 陈维军, 张俐勤   

  1. 华中农业大学食品科技学院; 嘉兴学院
  • 出版日期:2006-07-15 发布日期:2011-09-29

Study on Analysis of Main Glycosides from Siraitia grosvenorii Fruits with High-performance Liquid Chromatography

 ZHANG  Yun-Zhu, QI  Xiang-Yang, CHEN  Wei-Jun, ZHANG  Li-Qin   

  1. 1.College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2.Jiaxing College, Jiaxing 314000, China
  • Online:2006-07-15 Published:2011-09-29

摘要: 目的:探讨罗汉果皂甙的HPLC分析法,并对罗汉果中主要皂甙成分罗汉果皂甙V、11-O-罗汉果皂甙V、罗汉果皂甙IV以及赛门甙I的含量进行分析。方法:以C18柱为色谱柱,采用反相高效液相法分离分析罗汉果皂甙。结果:建立了罗汉果皂甙的HPLC分析条件,其色谱条件为:流动相,23%的乙腈水溶液;流速1ml/min;检测波长,210nm;柱温,室温;灵敏度,0.04AUFS。采用上述的液相分析方法分析四种罗汉果皂甙的含量,发现该方法简便、快速、准确、线性范围在0.5~13.75μg之间,且线性关系良好(R2≥0.99),加标回收率高(94.46%~105.05%),精密度良好(RSD=1.40%~3.31%)。结论:建立的HPLC方法可用于分析罗汉果及其制品中的罗汉果皂甙含量。

关键词: 高效液相色谱法, 罗汉果皂甙, 罗汉果

Abstract: Objective: To study the use of HPLC to assay the content of mogroside V, 11-oxo-mogroside, mogroside IV andSiamenoside I in Siraitia grosvenorii fruits. Methods: The RP-HPLC was used to determine the content of mogrosides. The analysis was carried on C18 column. Results: The conditions to establish the HPLC method for determination of mogrosides were: eluting solvent, acetonitrile-water, 23:77 (V/V); flowrate, 1.0ml/min; detection, 210nm; temperature, ambient and sensitivity setting, 0.04AUFS. The method is simple, easy and of good linear range (0.5~13.75μg) with the correlationship over 0.99. The recovery is 94.46%~105.05%, and the RSD 1.40%~3.31%. Conclusion: The HPLC method can be used to determine the content of mogrosides in Siraitia grosvenorii and its products.

Key words: HPLC, mogrosides, Siraitia grosvenorii