食品科学 ›› 2004, Vol. 25 ›› Issue (1): 171-173.

• 营养卫生 • 上一篇    下一篇

黑木耳多糖清除活性氧及保护线粒体

 李兴泰, 高明波   

  1. 大连民族学院生物工程系
  • 出版日期:2004-01-15 发布日期:2011-10-24

Effects of Auricularia auricula Polysaccharide on Scavenging Reactive Oxygen Speciesand Protecting Mitochondria

 LI  Xing-Tai, GAO  Ming-Bo   

  1. Department of Bioengineering, Dalian Nationalities University
  • Online:2004-01-15 Published:2011-10-24

摘要: 目的:观察黑木耳多糖(AAP)是否具有清除超氧阴离子(O-2•)、抗氧化、保护线粒体作用。方法:以Fe2+-L-半胱氨酸(L-Cys)体系体外诱发鼠肝、脑线粒体及匀浆脂质过氧化并导致线粒体肿胀。以还原型辅酶I-吩嗪硫酸甲酯-氮蓝四唑(NADH-PMS-NBT)系统产生O-2•,用分光光度法测定脂质过氧化产物丙二醛(MDA)含量、线粒体肿胀度和O-2•的清除情况。结果:黑木耳多糖可明显抑制MDA生成及线粒体肿胀,并能明显清除O-2•,且呈剂量效应关系。结论:黑木耳多糖具有清除超氧阴离子、抗氧化及保护线粒体功能。

关键词: 黑木耳多糖, 线粒体, 抗氧化剂, 活性氧

Abstract: Aim: To investigate if Auricularia auricula polysaccharide(AAP) would have the effects of scavenging superoxideanion(O-2•), antioxidation ,protecting mitochondria from injury. Methods: Lipid peroxidation and swelling of mouse liver andbrain mitochondria were induced by Fe2+-L-Cys system in vitro. Superoxide anion was generated by reduced nicotinamide adeninedinucleotide(NADH )- N-Methylphenazonium methyl sulfate(PMS)-4-Nitrobluetetrazolium chloride(NBT)system. Productof lipid peroxidation malondialdehyde(MDA) and swelling and superoxide anion production were determined spectrophotometrically.Results:AAP could inhibit MDA production,protect mitochondria from swelling,and scavenge O-2•obviously in a dose-dependent manner. Conclusion:AAP had the effects of scavenging O2-•, antioxidation and protecting mitochondria frominjury.

Key words: Auricularia auricula polysaccharide, mitochondria, antioxidant, reactive oxygen species.