黄桃水溶性多糖的提取和分离纯化

食品科学 ›› 2004, Vol. 25 ›› Issue (1): 81-84.

黄桃水溶性多糖的提取和分离纯化

陈留勇, 孟宪军, 孔秋莲, 宋立峰

沈阳农业大学食品学院，上海农业科学院农产品加工保鲜中心，辽宁经济职业技术学院生物应用工程系

出版日期:2004-01-15 发布日期:2011-10-24

Extraction Isolation and Purification of Water Soluble Polysaccharides from Peach

CHEN Liu-Yong, MENG Xian-Jun, KONG Qiu-Lian, SONG Li-Feng

1.Food college of ShenYang Agriculture University;2.Centre of the Procession andPreservation of Agriculture Product of ShangHai Academy of Agriculture Science;3.Department of Technology of Biological Appliance of Liaoning Technological Institute of Economic Vocation

Online:2004-01-15 Published:2011-10-24

摘要/Abstract

摘要： 采用水提醇沉法从黄桃果肉中提取出水溶性多糖,经去蛋白和色素,得到纯白色的黄桃水溶性多糖HTP,过DEAE-纤维素柱分别在水洗脱和盐洗脱部分得到HTP1和HTP2,HTP1和HTP2过Sephadex G-300凝胶柱,仍为单一对称峰,采用凝胶色谱法和纸层析鉴定纯度,表明为纯品。

关键词: 黄桃, 多糖, 分离, 纯化

Abstract: The water soluble polysaccharides of peach were extracted in hot water, then isolated by ethanol. The proteinin polysaccharides was removed by Sevag way and the pigment in polysaccharides was removed by H2O2. The water-solublepolysaccharides of peach which color was pure white were got. HTP were further purified to HTP1 and HTP2 with DEAEcolumn chromatography. HTP1 and HTP2 were purified with Sephadex-300 column chromatography respectively, howeverwere singular peak. The gel filtration chromatography and paper chromatography proved that HTP1 and HTP2 were purematerial.

Key words: peach, polysaccharides, isolation, purification

陈留勇, 孟宪军, 孔秋莲, 宋立峰. 黄桃水溶性多糖的提取和分离纯化[J]. 食品科学, 2004, 25(1): 81-84.

CHEN Liu-Yong, MENG Xian-Jun, KONG Qiu-Lian, SONG Li-Feng. Extraction Isolation and Purification of Water Soluble Polysaccharides from Peach[J]. FOOD SCIENCE, 2004, 25(1): 81-84.

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