食品科学 ›› 2004, Vol. 25 ›› Issue (1): 39-46.

• 基础研究 • 上一篇    下一篇

响应曲面法评价环境因子对肉品发酵过程中微生物的影响

 李宗军, 江汉湖   

  1. 湖南农业大学食品科技学院,南京农业大学食品科技学院
  • 出版日期:2004-01-15 发布日期:2011-10-24

Effect of Environment Factors on Microorganism and Its Evaluation with Response Surface Analysis During Meat Fermentation

 LI  Zong-Jun, JIANG  Han-Hu   

  1. 1.The College of Food Science and Technology, Hunan Agriculture University;2.The College of Food Science and Technology, Nanjing Agricultural University
  • Online:2004-01-15 Published:2011-10-24

摘要: 用响应曲面试验设计,研究了盐浓度、水分活度、发酵温度、发酵时间和糖浓度等环境因子对肉品发酵过程中的细菌总数、乳酸细菌、微球菌和酵母菌的影响,确定了有显著性影响的环境因子,建立了科学的数学模型。优化了传统酸肉的生产条件,最佳参数是:食盐8%,水分活度0.95,发酵温度25℃,发酵时间25d,白砂糖2%。并对最佳工艺发酵25d的产品与传统工艺发酵(自然发酵)90d的产品进行了感官鉴评,结果没有显著性差异,新工艺可行。用建立的数学模型对发酵过程中的微生物进行了预测,结果可靠。这为肉品发酵过程的控制,提高发酵肉制品的安全性,缩短生产周期提供了依据,也是微生物预报的重要基础。

关键词: 肉, 发酵肉制品, 预报微生物学, 响应曲面法

Abstract: Effect of environment factors, such as salt concentration, water activity, degree and time of fermentation, sugarconcentration etc. on total bacteria, lactic acid bacteria, microcococcus and yeast was studied with response surface experimentdesign. Significant environmental factors were assured and scientific mathematical models were founded. Conditions of the besttechnology of traditional fermented meat products--Nanx wudl were salt 8%, Aw 0.95, fermentation degree 25℃, fermentationtime 25d, sugar 2%.Sensory evaluation showed non-significant between the products produced by best technology for 25d andthat of natural fermentation for 90d, so the renovation techniques were successful. Applied the mathematical model to predictthe number of microorganism during fermentation, the result was credible. It gave some information to regulate fermentation,provide safety of fermented meat products and short production cycle, and also as important elements of prediction microbiology.

Key words: meat, fermented meat products, prediction microbiology, response curve surface design