食品科学 ›› 2004, Vol. 25 ›› Issue (3): 139-142.

• 分析检测 • 上一篇    下一篇

玉米须黄酮类测定方法的研究

 任顺成, 丁霄霖   

  1. 江南大学食品学院
  • 出版日期:2004-03-15 发布日期:2011-10-24

Study on Determination Methods of Flavonoids from Corn Silk

 REN  Shun-Cheng, DING  Xiao-Lin   

  1. School of Food Science and Technology, Southern Yangtze University
  • Online:2004-03-15 Published:2011-10-24

摘要: 本文选用多种黄酮类测定方法,通过对玉米须黄酮类提取液、单体及芦丁标样用不同的显色剂反应后,测定各反应液在200600nm范围内的吸收曲线,确定了适合玉米须黄酮类定量测定的波长,并对不同测定方法的定量结果进行了比较。结果表明,Al(NO3)3比色法不适合测定玉米须黄酮类的含量,而AlCl3比色法是一种简便、准确的方法,以芦丁为标样,可用于玉米须黄酮类含量的测定。

关键词: 玉米须, 黄酮类, 测定

Abstract: Several methods were selected to determine the content of flavonoids in corn silk, while satisfying wave length wasdecided by means of UV-visible photometer curves of flavonoids and its monomer in corn silk, as well as rutin at the range of200600nm after reacting with different regents , and comparison of experimental results were carried out. The results showedthat Al(NO3)3 colorimetry is not suitable to determine content of flavonoids in corn silk. While AlCl3 colorimetry is a simple,accuratemethod which can be used to determine content of corn silk flaovonoids with rutin as reference substance.

Key words: corn silk, flavonoids, determination