食品科学 ›› 2004, Vol. 25 ›› Issue (3): 43-45.

• 基础研究 • 上一篇    下一篇

红托竹荪多糖的提取分离及组成研究

 连宾, 郁建平   

  1. 贵州工业大学化学与生物工程学院,贵州大学生化营养研究所,重庆大学西南资源开发及环境灾害控制工程教育部重点实验室
  • 出版日期:2004-03-15 发布日期:2011-10-24

Study on the Polysaccharides Extraction from Mushroom Dictyophora rubrovalvata by TLC

 LIAN  Bin, YU  Jian-Ping   

  1. 1. Chemistry and Bio-engineering College, Guizhou University of Technology;2. Key Lab for the Exploitation of Southwestern Resources & the Evironmental Disaster Control EngineeringMinistry of Education;3. Institute of Biochemistry and Nutrition, Guizhou Univer-sity
  • Online:2004-03-15 Published:2011-10-24

摘要: 本文对红托竹荪(Dictyophora rubrovalvata)多糖的提取、纯化和单糖的组成成分进行了研究。红托竹荪子实体经热水提取、乙醇沉淀得多糖(Dr)粗品,多糖粗品经脱蛋白,乙醇分级沉淀、Sephadex G-15葡聚糖凝胶柱层析纯化得Dr-1,Dr-2,Dr-3三个级分,应用薄层层析技术,确定该三个级分均由半乳糖、葡萄糖、甘露糖和木糖组成。

关键词: 红托竹荪, 多糖, 分离, 纯化

Abstract: In this paper, the crude polysaccharides from the fruitbody of mushroom Dictyophora rubrovolota were obtainedby extracting with hot water and precipitating with alcohol. The free proteins were removed from crude polysaccharides, andthen the polysaccharides were isolated and purified by Sephadex G-15 gel column chromatography. Three pure polysaccharides(Dr-1, Dr-2 and Dr-3) were obtained by precipitating with different concentration alcohol. By using the TLC method, we provedthat the three fractions were all composed of galactose (Gal), glucose (Glu), mannose (Man) and xylose (Xyl).

Key words: Dictyophora rubrovolota, polysaccharides, isolation, purification, TLC (Thin-layer-chromafography)